Abstract | AIMS: To study the expression of p63, cytokeratin (CK) 5 and CK8/18 in invasive ductal carcinomas and their relationship with BRCA1 and other pathological and immunohistochemical features of clinical significance. METHODS AND RESULTS: Immunohistochemistry with the antibodies p63, CK5, CK8/18, BRCA1, oestrogen receptor, progesterone receptor, p53, c-erbB-2 and Ki67 was performed in 102 formalin-fixed paraffin-embedded samples of invasive ductal carcinomas. The CK5+ cases were submitted to a double-immunolabelling study with p63. There was a strong relationship between CK5 and p63 expression and both markers were associated with hormonal receptor-negative high-grade carcinomas with high proliferative rate. Furthermore, there was coexpression of CK5 and p63 in neoplastic cells, indicating that p63, like CK5, is a marker of the basal phenotype of breast cancer. There was a strong relationship between reduced expression of BRCA1 with both p63 and CK5 expression as well as an inverse correlation between p63 and CK8/18 expression, suggesting that loss of p63 expression is required for the transition between a basal to a luminal phenotype of breast carcinoma. CONCLUSIONS: Since p63 is thought to be a marker of stem cells and may act as an oncogene, our data support the idea that BRCA1 acts as stem cell regulator.
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Authors | A Ribeiro-Silva, L N Z Ramalho, S B Garcia, D F Brandão, F Chahud, S Zucoloto |
Journal | Histopathology
(Histopathology)
Vol. 47
Issue 5
Pg. 458-66
(Nov 2005)
ISSN: 0309-0167 [Print] England |
PMID | 16241993
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- BRCA1 Protein
- DNA-Binding Proteins
- Phosphoproteins
- TP63 protein, human
- Trans-Activators
- Transcription Factors
- Tumor Suppressor Proteins
- Keratins
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Topics |
- Adult
- Aged
- BRCA1 Protein
(biosynthesis)
- Breast Neoplasms
(metabolism, pathology)
- Carcinoma, Ductal, Breast
(metabolism, pathology)
- DNA-Binding Proteins
- Female
- Genes, Tumor Suppressor
- Humans
- Immunohistochemistry
- Keratins
(biosynthesis)
- Middle Aged
- Phosphoproteins
(biosynthesis)
- Trans-Activators
(biosynthesis)
- Transcription Factors
- Tumor Suppressor Proteins
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