Assembly of MHC class I molecules in ex vivo carcinoma cells induced by IFN-gamma or by a binding peptide.

Abstract

It has been reported that the assembly of MHC class I molecules in mutagenized cell lines could be induced by specific binding peptides. We have now demonstrated that the defect in assembly between heavy and light chains of class I molecules naturally occurred in tumor cells of one spontaneous ovarian carcinoma detected by one-dimensional isoelectric focusing of immunoprecipitates with anti-monomorphic class I MAb (W6/32) and by immunostaining with free heavy chain and beta 2m-specific MAbs. In vitro treatment of the tumor cells with IFN-gamma induced the assembly and surface expression of majority class I molecules (A2.1, B7, B15, Cw6, Cw7 out of A2.1, A2*, B7, B15, Cw6, Cw7). Moreover, assembly of A2 and Cw6 was induced by exposure of the tumor cells to a HLA A2-binding peptide K62 derived from influenza A matrix protein. Autologous blood T lymphocytes were activated in mixed lymphocyte-tumor cell culture (MLTC) by the IFN-gamma-treated but not by the unmanipulated tumor cells. Although activated lymphocytes damaged both IFN-gamma-treated and untreated tumor cells, the alpha class I MAb (W6/32) efficiently inhibited the lysis of IFN-gamma-treated targets, but not the untreated targets. These results indicate that the defect of MHC class I assembly may result in the escape of tumor cells from immune response.

• Authors: P Wang, F Vánky, Z Végh, U Persson, C Hising, E Klein
• Journal: Cellular immunology (Cell Immunol) Vol. 142 Issue 2 Pg. 296-302 (Jul 1992) ISSN: 0008-8749 [Print] Netherlands
• PMID: 1623553 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HLA-A2 Antigen
• Histocompatibility Antigens Class I
• Intercellular Signaling Peptides and Proteins
• Peptides
• A2-binding peptide
• Interferon-gamma

Topics

• Amino Acid Sequence
• Female
• HLA-A2 Antigen (analysis, biosynthesis)
• Histocompatibility Antigens Class I (analysis, biosynthesis)
• Humans
• Intercellular Signaling Peptides and Proteins
• Interferon-gamma (pharmacology)
• Lymphocyte Activation (immunology)
• Molecular Sequence Data
• Ovarian Neoplasms (immunology)
• Peptides (pharmacology)
• Tumor Cells, Cultured (drug effects, immunology)