Abstract | OBJECTIVE: METHODS:
Acid- ethanol extracts of human synovium were examined for growth-promoting activity by measuring formation of 14C-glycosaminoglycan (14C-GAG) and 3H-DNA in synovial cell cultures; PGE2 was measured by enzyme immunoassay, and CTAP-III isoforms were identified by Western blotting of extracted proteins separated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis. Growth-promoting activity of CTAP-III and other cytokines was tested in synovial cultures treated with the agonists singly and in binary combination, by measuring changes in synthesis of 14C-GAG and 3H-DNA. RESULTS: CONCLUSIONS: The results suggest that, in addition to endogenous factors, CTAP-III and other platelet-derived cytokines may play roles in regulating synovial cell metabolism in RA and OA, and that combinations of growth factors may be more significant than single agents in amplification or suppression of important cell functions.
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Authors | C W Castor, E M Smith, P A Hossler, M C Bignall, B P Aaron |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 35
Issue 7
Pg. 783-93
(Jul 1992)
ISSN: 0004-3591 [Print] United States |
PMID | 1622417
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Growth Substances
- Peptides
- connective tissue-activating peptide
- Dinoprostone
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Topics |
- Arthritis, Rheumatoid
(physiopathology)
- Blotting, Western
- Cells, Cultured
- Dinoprostone
(chemistry)
- Growth Substances
(physiology)
- Humans
- Osteoarthritis
(physiopathology)
- Peptides
(analysis, chemistry)
- Synovial Fluid
(chemistry, metabolism)
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