Abstract | OBJECTIVE: METHODS: Experiment 1: Sixty Wistar rats were divided into three groups. Rats of Group A (n = 20) underwent intraperitoneal administration of placebo as a control group, rats of Group B (n = 20) first underwent intraperitoneal administration of 100 mg/kg ONO-4057 and rats of Group C (n = 20) first underwent intraperitoneal administration of 300 mg/kg ONO-4057 daily from day 3 before anti-Thy-1 antibody (OX7) injection to day 14 after OX7 injection, respectively. Experiment 2: Forty rats were divided into two groups. ONO-group (n = 20) was treated with 300 mg/kg BW of ONO-4057 and placebo-group (n = 20) with placebo daily from days 1 to 13 after OX7 injection. Urine and blood samples were collected and the kidneys were extirpated from five rats of each group sacrificed at 3 h, 24 h, day 7 or day 14 after the injection of OX7 in both experiments. Urinary protein excretion, renal function and pathological findings were analysed in each group of both experiments. RESULTS: (1) Glomerular infiltration by polymorphonuclear leucocytes (PMNs) and macrophages at 3 h was less in Groups B and C than in Group A, and matrix scores at day 7 were lower in Groups B and C than in Group A. Injury scores did not differ among the groups. (2) Urinary protein excretion at day 7 was less in Group C than in Group A. (3) Neither pathological findings nor urinary protein excretion differed between ONO-group and placebo-group. CONCLUSION: These results suggest that LTB4 is associated not with the pathogenesis of complement-dependent mesangial cell lysis but with that of mesangial proliferative change in anti-Thy-1 nephritis.
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Authors | Yukihiko Kawasaki, Mieko Tanji, Kei Takano, Yutaka Fukuda, Masato Isome, Ruriko Nozawa, Hitoshi Suzuki, Mitsuaki Hosoya |
Journal | Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
(Nephrol Dial Transplant)
Vol. 20
Issue 12
Pg. 2697-703
(Dec 2005)
ISSN: 0931-0509 [Print] England |
PMID | 16221713
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Immunosuppressive Agents
- Isoantibodies
- Phenylpropionates
- Receptors, Leukotriene B4
- anti-Thy antibody
- ONO-LB 457
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Topics |
- Animals
- Antibodies, Monoclonal
(toxicity)
- Disease Models, Animal
- Female
- Glomerulonephritis, Membranoproliferative
(chemically induced, drug therapy, metabolism)
- Immunohistochemistry
- Immunosuppressive Agents
(pharmacology)
- Isoantibodies
(toxicity)
- Kidney Glomerulus
(drug effects, immunology, pathology)
- Phenylpropionates
(pharmacology)
- Rats
- Rats, Wistar
- Receptors, Leukotriene B4
(antagonists & inhibitors)
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