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[Enhancive effect of histone deacetylase inhibitor trichostatin a on transfection efficiency of adenovirus in ovarian carcinoma cell line A2780].

AbstractBACKGROUND & OBJECTIVE:
The presence of Coxsackie and adenovirus receptor (CAR) on target cell surface is required for efficient adenovirus transfection; lack or down-regulated expression of CAR on cancer cells is the main cause of inefficiency of adenovirus-based gene therapy. This study was to evaluate enhancive effect of trichostatin A (TSA), a histone deacetylase (HDAC) inhibitor, on the transfection efficiency of adenovirus in ovarian carcinoma cell line A2780, and explore its possible application to adenovirus-based gene therapy.
METHODS:
mRNA and protein levels of CAR on A2780 cells were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot before and after treatment of TSA. Transfection efficiency of adenovirus was valued by flow cytometry (FCM). In vitro antitumor effect of adenovirus/thymidine kinase (ADV/TK) was detected by MTT assay.
RESULTS:
After treatment of TSA, mRNA and protein levels of CAR on A2780 cells were obviously increased. Transfection rates of adenovirus were (1.24+/-0.14)% in untreated group, (7.58+/-0.32)% in 5 nmol/L of TSA treated group, and (7.94+/-0.28)% in 100 nmol/L of TSA treated groups. In vitro antitumor effect of ADV/TK was 4-10 folds in TSA (5 or 100 nmol/L) treated groups compared with that in untreated group.
CONCLUSION:
TSA could enhance transfection efficiency of adenovirus in ovarian carcinoma cells, and may be useful in gene therapy for ovarian carcinoma.
AuthorsGang Chen, Bei-Bei Wang, Fu-Jun Li, De-Yan Liu, Jian-Feng Zhou, Yun-Ping Lu, Ding Ma
JournalAi zheng = Aizheng = Chinese journal of cancer (Ai Zheng) Vol. 24 Issue 10 Pg. 1196-200 (Oct 2005) China
PMID16219132 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • CLMP protein, human
  • Coxsackie and Adenovirus Receptor-Like Membrane Protein
  • Histone Deacetylase Inhibitors
  • Hydroxamic Acids
  • RNA, Messenger
  • Receptors, Virus
  • trichostatin A
  • Thymidine Kinase
Topics
  • Adenoviridae (genetics, physiology)
  • Cell Line, Tumor
  • Coxsackie and Adenovirus Receptor-Like Membrane Protein
  • Female
  • Histone Deacetylase Inhibitors
  • Humans
  • Hydroxamic Acids (pharmacology)
  • Ovarian Neoplasms (metabolism, pathology, virology)
  • RNA, Messenger (biosynthesis, genetics)
  • Receptors, Virus (biosynthesis, genetics)
  • Thymidine Kinase (pharmacology)
  • Transfection

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