Antisense-induced blockade of GATA-3 expression could inhibit Th2 excursion of tumor cells in vitro and in vivo.

Abstract

Previous studies have shown that tumor cells predominantly express Th2 type cytokines and transcription factors. GATA-3, as a Th2-specific transcription factor, plays a central role in positive-regulating Th2 development. So whether the expression of GATA-3 in tumor cells has any effect on tumor development is a question of interest. In the present study, we inhibited the expression of GATA-3 in tumor cells through antisense RNA blockade technique, and observed its effects on tumor in vitro and in vivo. Our results showed that antisense GATA-3 treatment could inhibit the expression of TNF-alpha and Th2 cytokines in tumor cells, and antisense-induced blockade of GATA-3 could also depress tumor growth in tumor-bearing mice. We suggest that the ratio of T-bet/GATA-3 can be evaluated as a more important marker of the status of Th1/Th2 type. And our results might provide some evidence about the molecular regulatory mechanisms in tumor cell development.

• Authors: Dongzhu Yao, Xiaojun Zhang, Haiming Wei, Zhigang Tian
• Journal: Cellular & molecular immunology (Cell Mol Immunol) Vol. 2 Issue 3 Pg. 189-96 (Jun 2005) ISSN: 1672-7681 [Print] China
• PMID: 16212886 (Publication Type: Journal Article)

Chemical References

• Cytokines
• GATA3 Transcription Factor
• RNA, Antisense
• Tumor Necrosis Factor-alpha

Topics

• Animals
• Cell Line, Tumor
• Cloning, Molecular
• Cytokines (metabolism)
• GATA3 Transcription Factor (biosynthesis, deficiency, genetics, metabolism)
• Gene Expression Regulation
• Gene Expression Regulation, Neoplastic
• Humans
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• Neoplasms (immunology, metabolism, pathology)
• RNA, Antisense (genetics, metabolism)
• Spleen (metabolism)
• Th1 Cells (immunology, metabolism)
• Th2 Cells (immunology, metabolism)
• Transfection
• Tumor Necrosis Factor-alpha (metabolism)