Anti-inflammatory and antitumor-promoting effects of the triterpene acids from the leaves of Eriobotrya japonica.

Abstract

Sixteen triterpene acids, viz., five of the oleanane-type (1-5), nine of the ursane-type (6-14), and two of the lupane-type (15, 16), were isolated and identified from the ethyl acetate-soluble fraction of the methanol extract of the leaves of loquat, Eriobotrya japonica LINDL. (Rosaceae). Twelve of these compounds, 1-4, 6, 8-13, and 15, were evaluated for their inhibitory effects on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation (1 microg/ear) in mice. All the compounds tested showed a marked anti-inflammatory effect, with a 50% inhibitory dose (ID50) of 0.03-0.43 mg per ear. In addition, an evaluation against the Epstein-Barr virus early antigen (EBV-EA) activation induced by TPA for all of the compounds, 12 and 13 showed potent inhibitory effects on EBV-EA induction. Furthermore, euscaphic acid (12) exhibited marked antitumor-promoting activity in an in vivo two-stage carcinogenesis test of mouse tumor by using 7,12-dimethylbenz[a]anthracene (DMBA) as an initiator and TPA as a promoter.

• Authors: Norihiro Banno, Toshihiro Akihisa, Harukuni Tokuda, Ken Yasukawa, Yosuke Taguchi, Hiroyuki Akazawa, Motohiko Ukiya, Yumiko Kimura, Takashi Suzuki, Hoyoku Nishino
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 28 Issue 10 Pg. 1995-9 (Oct 2005) ISSN: 0918-6158 [Print] Japan
• PMID: 16204964 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Anticarcinogenic Agents
• Triterpenes
• Tetradecanoylphorbol Acetate

Topics

• Animals
• Anti-Inflammatory Agents (isolation & purification, pharmacology)
• Anticarcinogenic Agents (isolation & purification, pharmacology)
• Chromatography, High Pressure Liquid
• Eriobotrya (chemistry)
• Magnetic Resonance Spectroscopy
• Mice
• Papilloma (prevention & control)
• Plant Leaves (chemistry)
• Skin Neoplasms (prevention & control)
• Spectrometry, Mass, Electrospray Ionization
• Tetradecanoylphorbol Acetate (pharmacology)
• Triterpenes (isolation & purification, pharmacology)