The release of soluble VAP-1/SSAO by 3T3-L1 adipocytes is stimulated by isoproterenol and low concentrations of TNFalpha.

Abstract

Plasma level of the protein VAP-1/SSAO (Vascular Adhesion Protein-1/Semicarbazide-Sensitive Amine Oxidase) is increased in diabetes and/or obesity and may be related to vascular complications associated to these pathologies. The aim of this work was to complete a preceding study where we described the role played by some hormones or metabolites, implicated in diabetes and/or obesity, in the regulation of the release of VAP-1/SSAO by 3T3-L1 adipocytes. Here we focused on the previously observed effect produced by TNFalpha in the release of VAP-1/SSAO and studied the effect of a beta-adrenergic compound, isoproterenol. Both compounds stimulated the release of VAP-1/SSAO to the culture medium but had a different effect on the VAP-1/SSAO membrane form. While TNFalpha produced a decrease on VAP-1/SSAO membrane form content, isoproterenol did not modify it. We thus observed two different ways of regulation of the release of VAP-1/SSAO by 3T3-L1 adipocytes by metabolites implicated in diabetes and adipose tissue physiopathology. Our work permits a better understanding of this increased plasma VAP-1/SSAO levels observed in diabetes.

• Authors: S García-Vicente, A Abella, N Viguerie, A Ros-Baró, M Camps, X Testar, M Palacín, A Zorzano, L Marti
• Journal: Journal of physiology and biochemistry (J Physiol Biochem) Vol. 61 Issue 2 Pg. 395-401 (Jun 2005) ISSN: 1138-7548 [Print] Spain
• PMID: 16180338 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adrenergic beta-Agonists
• Cell Adhesion Molecules
• Tumor Necrosis Factor-alpha
• Amine Oxidase (Copper-Containing)
• semicarbazide-sensitive amine oxidase-vascular adhesion protein-1, mouse
• Isoproterenol

Topics

• 3T3-L1 Cells
• Adipocytes (drug effects, enzymology, metabolism)
• Adrenergic beta-Agonists (pharmacology)
• Amine Oxidase (Copper-Containing) (analysis, metabolism)
• Animals
• Blotting, Western
• Cell Adhesion Molecules (metabolism)
• Cell Culture Techniques
• Cell Fractionation
• Dose-Response Relationship, Drug
• Electrophoresis, Polyacrylamide Gel
• Isoproterenol (pharmacology)
• Mice
• Solubility
• Tumor Necrosis Factor-alpha (pharmacology)