[Expression of adrenomedullin and its receptor mRNA in the tissues of normal adrenal medulla and pheochromocytoma].

Abstract

OBJECTIVE:

To investigate the expression of human adrenomedullin (ADM) and its receptor-receptor activity modifying protein 2/calcitonin receptor-like receptor (RAMP2/CRLR) mRNA in the tissues of normal adrenal medulla and pheochromocytoma.

METHODS:

Total RNA was extracted from normal adrenal medulla and pheochromocytomas. The expression of ADM and RAMP2/CRLR mRNA were studied by reverse transcription-polymerase chain reaction. The ratios of ADM/GAPDH, RAMP2/ GAPDH, CRLR/GAPDH were used to evaluate the expression levels of ADM, RAMP2 and CRLR mRNA.

RESULTS:

Expressions of ADM and its receptor- RAMP2/CRLR mRNA were detected in normal adrenal medulla and pheochromocytoma tissues. ADM/GAPDH were 0.48+/-0.09 and 0.75+/-0.24, RAMP2/ GAPDH 0.79+/-0.12 and 1.29+/-0.30, CRLR/GAPDH 0.40+/-0.08 and 0.87+/-0.22 in normal adrenal medulla and pheochromocytomas, respectively (P < 0.05).

CONCLUSION:

ADM exerts a possible autocrine or paracrine effect in the adrenal. ADM may be involved in the pathogenesis of pheochromocytoma.

• Authors: Dong-Mei Liu, Zheng-Pei Zeng, Han-Zhong Li, Xin-Rong Fan, Guo-Qiang Liu, Wei-Gang Yan, An-Li Tong, Xin Zheng
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 27 Issue 4 Pg. 452-6 (Aug 2005) ISSN: 1000-503X [Print] China
• PMID: 16178438 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Intracellular Signaling Peptides and Proteins
• Membrane Proteins
• Peptides
• RAMP2 protein, human
• RNA, Messenger
• Receptor Activity-Modifying Protein 2
• Receptor Activity-Modifying Proteins
• Receptors, Adrenomedullin
• Receptors, Calcitonin
• Receptors, Peptide
• Adrenomedullin
• Calcitonin Gene-Related Peptide

Topics

• Adrenal Gland Neoplasms (metabolism)
• Adrenal Medulla (metabolism)
• Adrenomedullin
• Adult
• Calcitonin Gene-Related Peptide (biosynthesis, genetics)
• Female
• Humans
• Intracellular Signaling Peptides and Proteins (genetics)
• Male
• Membrane Proteins (biosynthesis, genetics)
• Middle Aged
• Peptides (genetics, metabolism)
• Pheochromocytoma (metabolism)
• RNA, Messenger (biosynthesis, genetics)
• Receptor Activity-Modifying Protein 2
• Receptor Activity-Modifying Proteins
• Receptors, Adrenomedullin
• Receptors, Calcitonin (biosynthesis, genetics)
• Receptors, Peptide (metabolism)