Abstract | BACKGROUND: METHODS: RT-PCR, western blotting and immunohistochemical techniques were used to examine EphB4 expression and protein levels in human prostate cancer cell lines LNCaP, DU145 and PC3. Immunohistochemistry was also used to examine localisation of EphB4 in tissue samples from 15 patients with prostate carcinomas. RESULTS: All three prostate cancer cell lines expressed the EphB4 gene and protein. EphB4 immunoreactivity in vivo was significantly greater in human prostate cancers as compared with matched normal prostate epithelium and there appeared to be a trend towards increased expression with higher grade disease. CONCLUSION:
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Authors | Yen-Ching Lee, Janeanne R Perren, Evelyn L Douglas, Michael P Raynor, Maria A Bartley, Peter G Bardy, Sally-Anne Stephenson |
Journal | BMC cancer
(BMC Cancer)
Vol. 5
Pg. 119
(Sep 20 2005)
ISSN: 1471-2407 [Electronic] England |
PMID | 16171530
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Receptor, EphB4
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Topics |
- Antineoplastic Agents
(pharmacology)
- Blotting, Western
- Carcinoma
(enzymology, metabolism)
- Cell Line, Tumor
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Male
- Microscopy, Fluorescence
- Prostatic Neoplasms
(enzymology, metabolism)
- Receptor, EphB4
(biosynthesis)
- Reverse Transcriptase Polymerase Chain Reaction
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