99mTc-labeled 1-thio-beta-D-glucose as a new tumor-seeking agent: synthesis and tumor cell uptake assay.

Abstract

99mTc-labeled 1-thio-beta-D-glucose (99mTc-1-TG) was synthesized under different ligand concentrations. 1-Thio-beta-D-glucose (1-TG) showed different chemical properties and higher stability at acid pH as compared to neutral and basic pH. Under different ligand concentrations, 99mTc-1-TG was obtained with high labeling efficiency of >97%, but the HPLC chromatogram varied with the ligand concentration. The results of a tumor cell uptake assay also depended on the ligand concentration. With the lowest ligand concentration tested (0.5 mg/ml of 1-TG), we obtained the highest uptake. With different glucose and insulin concentrations, 99mTc-1-TG uptake was not significantly different from that of [18F]FDG.

• Authors: Seung Jun Oh, Jin-Sook Ryu, Eun-Jin Yoon, Mun Sun Bae, Sun Joo Choi, Kyung Bae Park, Dae Hyuk Moon
• Journal: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine (Appl Radiat Isot) Vol. 64 Issue 2 Pg. 207-15 (Feb 2006) ISSN: 0969-8043 [Print] England
• PMID: 16168660 (Publication Type: Journal Article)

Chemical References

• Radiopharmaceuticals
• Technetium
• 1-thioglucose
• Glucose

Topics

• Brain Neoplasms (diagnostic imaging, metabolism)
• Cell Line, Tumor
• Chromatography, High Pressure Liquid
• Colorectal Neoplasms (diagnostic imaging, metabolism)
• Glucose (analogs & derivatives, chemistry, pharmacokinetics)
• Humans
• Hydrogen-Ion Concentration
• Isotope Labeling (methods)
• Lung Neoplasms (diagnostic imaging, metabolism)
• Radionuclide Imaging
• Radiopharmaceuticals (chemical synthesis, pharmacokinetics)
• Technetium (chemistry)