Abstract | PURPOSE: EXPERIMENTAL DESIGN: Employing two-dimensional gel electrophoresis and mass spectrometry, we analyzed 16 matched colorectal cancer and adjacent normal tissue samples. Proteins found to be elevated in cancer tissue were further validated by generating antibodies which were used for immunoblotting of tissue samples and for the development of highly sensitive immunoassays for assessment of serum samples. RESULTS: CONCLUSIONS: It is proposed that NNMT serum levels may have significance in the early detection and in the management of patients with colorectal cancer.
|
Authors | Markus Roessler, Wolfgang Rollinger, Stefan Palme, Marie-Luise Hagmann, Peter Berndt, Alfred M Engel, Bernd Schneidinger, Michael Pfeffer, Herbert Andres, Johann Karl, Heinz Bodenmüller, Josef Rüschoff, Thomas Henkel, Gerhard Rohr, Siegbert Rossol, Wolfgang Rösch, Hanno Langen, Werner Zolg, Michael Tacke |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 11
Issue 18
Pg. 6550-7
(Sep 15 2005)
ISSN: 1078-0432 [Print] United States |
PMID | 16166432
(Publication Type: Journal Article)
|
Chemical References |
- Biomarkers, Tumor
- Blood Proteins
- Proteome
- Methyltransferases
- NNMT protein, human
- Nicotinamide N-Methyltransferase
|
Topics |
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
(blood)
- Blood Proteins
(analysis)
- Colorectal Neoplasms
(blood, diagnosis)
- Electrophoresis, Gel, Two-Dimensional
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Male
- Methyltransferases
(blood)
- Middle Aged
- Nicotinamide N-Methyltransferase
- Proteome
(analysis)
- Sensitivity and Specificity
|