N-Acetylcysteine inhibit the translocation of mixed lineage kinase-3 from cytosol to plasma membrane during transient brain ischemia in rat hippocampus.

Abstract

Mixed lineage kinase-3 (MLK3) is a recently described member of the MLK subfamily of Ser/Thr protein kinases that interacts with mitogen-activated protein kinase (MAPK) pathways. In this study, we investigated the translocation of MLK3 during transient cerebral ischemia in rat hippocampus. Transient brain ischemia was induced by the four-vessel occlusion in Sprague-Dawley rats. Our data show that MLK3 can translocate from cytosolic fraction to the membrane fraction during ischemia and the increased MLK3 in the membrane fraction bind to postsynaptic density protein 95 (PSD-95). The antioxidant N-acetylcysteine (NAC) could inhibit the translocation of MLK3 from cytosolic fraction to the membrane fraction and decrease the interactions of MLK3 and PSD-95 in the membrane fraction. Consequently, these results indicate that reactive oxygen species (ROS) was closely associated with MLK3 translocation induced by transient global ischemia in rat hippocampus.

• Authors: Dong-Sheng Pei, Qiu-Hua Guan, Ya-Feng Sun, Qing-Xiu Zhang, Tian-Le Xu, Guang-Yi Zhang
• Journal: Neuroscience letters (Neurosci Lett) Vol. 391 Issue 1-2 Pg. 38-42 (Dec 31 2005) ISSN: 0304-3940 [Print] Ireland
• PMID: 16154687 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Reactive Oxygen Species
• MAP Kinase Kinase Kinases
• mitogen-activated protein kinase kinase kinase 11
• Acetylcysteine

Topics

• Acetylcysteine (administration & dosage)
• Animals
• Brain Ischemia (metabolism)
• Cell Membrane (drug effects, metabolism)
• Cytosol (drug effects, metabolism)
• Dose-Response Relationship, Drug
• Hippocampus (drug effects, metabolism)
• MAP Kinase Kinase Kinases (metabolism)
• Male
• Protein Transport (drug effects)
• Rats
• Rats, Sprague-Dawley
• Reactive Oxygen Species (metabolism)