Abstract |
Antiangiogenic activity of curcumin on the tumor neogenesis was investigated by evaluating the density of neocapillaries induced by Hepatocellular carcinoma cells (HepG2) in mice, using intravital fluorescence videomicroscopy. Male BALB/c nude mice (20-25 g) were used, and a dorsal skin-fold chamber was implanted. HepG2 (30 microl of 2 x 10(6) cells) were inoculated on the upper surface of the skin within the chamber. The mice were divided into two groups as follows. Dimethyl sulfoxide solution (0.1%) was fed (HepG2 group, n=5) or curcumin solution (3000 mg/kg bw) was fed oral daily (HepG2-Cur group, n=5), one day after the inoculation of HepG. On days 7 and 14 post- tumor-inoculation, the tumor microvasculature was visualized by injecting 0.1 ml of 0.5% rhodamine B isothiocyanate-labeled dextran intravenously, and observed under an intravital fluorescence videomicroscope. Based on the recorded videoimage, the tumor neocapillary density and microvasculature were evaluated using a digital image analysis and correlated with the tumor area. The image analysis demonstrated that in the HepG2-group the neocapillary densities were significantly increased on day 7, and day 14, compared to the aged-matched Sham-group (P<0.05). In the HepG2-Cur group, the increase of tumor neocapillary density was attenuated significantly. It was suggested that high dose of curcumin might be an effective anti-angiogenic drug in the treatment against tumor.
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Authors | P Yoysungnoen, P Wirachwong, P Bhattarakosol, H Niimi, S Patumraj |
Journal | Clinical hemorheology and microcirculation
(Clin Hemorheol Microcirc)
Vol. 33
Issue 2
Pg. 127-35
( 2005)
ISSN: 1386-0291 [Print] Netherlands |
PMID | 16151260
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Angiogenesis Inhibitors
- Curcumin
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Topics |
- Angiogenesis Inhibitors
(administration & dosage, pharmacology)
- Animals
- Carcinoma, Hepatocellular
(blood supply, drug therapy, pathology)
- Curcumin
(administration & dosage, pharmacology)
- Drug Evaluation, Preclinical
- Humans
- Male
- Mice
- Mice, Nude
- Microscopy, Video
- Neoplasm Transplantation
- Neovascularization, Pathologic
(drug therapy)
- Transplantation, Heterologous
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