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Two nucleotides next to the anticodon of cytoplasmic rat tRNA(Asp) are likely generated by RNA editing.

Abstract
The nucleotide sequences of major cytoplasmic tRNA(Asp) from rat liver and rat ascites hepatoma comprise a U32 and C33 next to the anticodon as was confirmed by different procedures. Additionally we identified a tRNA(Asp) with C32 and U33 in a minor proportion. We have shown earlier that the tRNA(Asp) gene is part of a cluster of tRNA genes which is amplified at least ten times in the rat nuclear genome. Six independent isolated clones display identical sequences in the coding region of the tRNA(Asp) gene which differ from tRNA(Asp) in having C32 and T33. Using a combination of single-strand conformation polymorphism (SSCP) analyses and direct sequencing of polymerase chain reaction (PCR) products we have now demonstrated that no variant allele of the tRNA(Asp) gene with T32 and C33 exists in the rat genome. Together with the RNA sequencing data these findings strongly indicate that major rat tRNA(Asp) is generated by post-transcriptional pyrimidine transitions at positions 32 and 33 and that the minor tRNA(Asp) is its unedited precursor.
AuthorsH Beier, M C Lee, T Sekiya, Y Kuchino, S Nishimura
JournalNucleic acids research (Nucleic Acids Res) Vol. 20 Issue 11 Pg. 2679-83 (Jun 11 1992) ISSN: 0305-1048 [Print] England
PMID1614853 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anticodon
  • Oligodeoxyribonucleotides
  • RNA, Transfer, Asp
Topics
  • Animals
  • Anticodon
  • Base Sequence
  • Liver
  • Molecular Sequence Data
  • Molecular Structure
  • Multigene Family
  • Nucleic Acid Conformation
  • Oligodeoxyribonucleotides (chemistry)
  • Polymerase Chain Reaction
  • Polymorphism, Genetic
  • RNA Processing, Post-Transcriptional
  • RNA, Transfer, Asp (genetics)
  • Rats

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