Genomic cloning and characterization of a Pto-like gene SsPto-2 from Solanum surattense.

Abstract

A Pto-like gene (designated as SsPto-2) was isolated from Solanum surattense by using genomic walker technology which encoded a cytoplasmically localized serine-threonine protein kinase. Analysis of the 2365 bp segment revealed a gene including a 905 bp 5' flanking region, a 924 bp open reading frame (ORF) and a 536 bp 3' flanking region. The deduced amino acid sequence of the SsPto-2 gene shared high homology with other known Ptos. The deduced SsPto-2 protein contained no signal peptide with a calculated molecular weight of 34.61 kDa. The analysis of SsPto-2 promoter region and terminator region was also presented. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that SsPto-2 transcripts were up-regulated by defense-related factors such as gibberellic acid (GA(3)), salicylic acid (SA) and down-regulated by darkness. The cloning of the SsPto-2 gene will allow us to further study its potential role in disease resistance.

• Authors: Beibei Huang, Xiaojun Liu, Xinglong Wang, Yan Pi, Hainian Zeng, Juan Lin, Jiong Fei, Xiaofen Sun, Kexuan Tang
• Journal: DNA sequence : the journal of DNA sequencing and mapping (DNA Seq) Vol. 16 Issue 4 Pg. 277-87 (Aug 2005) ISSN: 1042-5179 [Print] England
• PMID: 16147887 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Gibberellins
• gibberellic acid
• Salicylic Acid

Topics

• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular (methods)
• Darkness
• Gene Expression Regulation, Plant
• Genes, Plant
• Gibberellins (pharmacology)
• Molecular Sequence Data
• Reverse Transcriptase Polymerase Chain Reaction
• Salicylic Acid (pharmacology)
• Sequence Alignment
• Sequence Analysis, DNA
• Solanum (chemistry, classification, genetics)