Abstract |
This study was carried out to investigate alterations of neurofilament 200 kDa (NF-200) and its polyphosphorylation form (RT97) immunoreactivity and protein content in the main olfactory bulb (MOB) after 5 min of transient forebrain ischemia in gerbils. In the sham-operated group, weak NF-200 immunoreactivity was detectable in a few somata of mitral cells, which projected weak NF-200-immunoreactive processes to the external plexiform layer (EPL). At 1-5 days after ischemia, strong NF-200 and RT97 immunoreactivity was shown by the mitral cell processes; however, somata of mitral cells did not show NF-200 immunoreactivity. At this time point, strong NF-200-immunoreactive mitral cell processes ran to the EPL and glomerular layer (GL). Thereafter, NF-200 and RT97 immunoreactivity was decreased up to 30 days after ischemia. In the 15 days post-ischemic group, the distribution pattern of NF-200 and RT97 immunoreactivity was slightly lower than that in the 1-5 days post-ischemic groups. In the 30 days post-ischemic group, moderate NF-200 and RT97 immunoreactivity was found in the mitral cells processes, but the immunoreactivity in the EPL and GL nearly disappeared. A Western blot study showed a pattern of NF-200 and RT97 expression at all post-ischemic time points similar to that of immunohistochemistry after ischemia. This result indicates that NF-200 and RT97 accumulates in injured mitral cell processes a few days after transient ischemia, which suggests that the axonal transport in the MOB may be disturbed during this period after transient ischemia.
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Authors | In Koo Hwang, Un-San Koh, Jae Chul Lee, Ki-Yeon Yoo, Ji-Hyun Song, Ju-Young Jung, Young Sam Nam, In Se Lee, Tae-Cheon Kang, Moo Ho Won |
Journal | Journal of the neurological sciences
(J Neurol Sci)
Vol. 239
Issue 1
Pg. 59-66
(Dec 15 2005)
ISSN: 0022-510X [Print] Netherlands |
PMID | 16140342
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Neurofilament Proteins
- Proteins
- neurofilament protein H
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Topics |
- Animals
- Axonal Transport
(physiology)
- Axons
(immunology, pathology)
- Blotting, Western
- Brain Ischemia
(metabolism, physiopathology)
- Cytoskeleton
(metabolism, pathology)
- Disease Models, Animal
- Gerbillinae
- Immunohistochemistry
- Ischemic Attack, Transient
(metabolism, physiopathology)
- Male
- Neurofilament Proteins
(metabolism)
- Neurons
(immunology, pathology)
- Neuropil
(immunology, pathology)
- Olfactory Bulb
(metabolism, pathology, physiopathology)
- Olfactory Pathways
(immunology, pathology, physiopathology)
- Phosphorylation
- Proteins
(analysis, metabolism)
- Time Factors
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