Abstract |
To investigate the role of Salmonella and Yersinia in the pathogenesis of spondyloarthropathies and rheumatoid arthritis synovial specimens from 92 patients were analysed for the presence of bacterial DNA with the use of polymerase chain reaction and for the presence of lipopolysaccharide and enterobacterial common antigen (ECA) with the use of Dot-ELISA. In addition, peripheral blood samples were available for PCR analysis from 68 patients. Salmonella and Yersinia chromosomal DNA was not found in any of the synovial specimens and blood samples from the patients. All of the synovial fluids were also culture-negative. Salmonella LPS antigens were observed in 8 (8.6%), Yersinia in 20 (21.7%) and ECA antigens in 32 (34.9%) synovial specimens. Our findings revealed the presence of bacterial degradation products, but not bacteria from the genus Salmonella and Yersinia or their DNA in the synovial fluid or blood of patients with spondyloarthropathies and rheumatoid arthritis.
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Authors | Waldemar Rastawicki, Marek Jagielski, Rafał Gierczyński, Halina Garwolińska, Brygida Kwiatkowska, Anna Filipowicz-Sosnowska |
Journal | Medycyna doswiadczalna i mikrobiologia
(Med Dosw Mikrobiol)
Vol. 57
Issue 2
Pg. 131-42
( 2005)
ISSN: 0025-8601 [Print] Poland |
Vernacular Title | Udział pałeczek z rodzaju Salmonella i Yersinia w patogenezie zapalenia stawów ze szczególnym uwzglednieniem spondyloartropatii i reumatoidalnego zapalenia stawów. I. Wystepowanie pałeczek z rodzaju Salmonella i Yersinia, fragmentów ich genów, lipopolisacharydów oraz antygenu ECA w płynie stawowym badź we krwi osób z zapaleniem stawów. |
PMID | 16134385
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Antigens, Bacterial
- DNA, Bacterial
- Lipopolysaccharides
- enterobacterial common antigen
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Topics |
- Antigens, Bacterial
(analysis)
- Arthritis, Rheumatoid
(microbiology)
- Blood
(microbiology)
- DNA, Bacterial
(isolation & purification)
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Lipopolysaccharides
(analysis)
- Male
- Salmonella
(isolation & purification)
- Spondylarthropathies
(microbiology)
- Synovial Fluid
(microbiology)
- Yersinia
(isolation & purification)
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