Immediate up-regulation of the calcium-binding protein S100P and its involvement in the cytokinin-induced differentiation of human myeloid leukemia cells.

Abstract

Cytokinins are important purine derivatives that act as redifferentiation-inducing hormones to control many processes in plants. Cytokinins such as isopentenyladenine (IPA) and kinetin are very effective at inducing the granulocytic differentiation of human myeloid leukemia HL-60 cells. We examined the gene expression profiles associated with exposure to IPA using cDNA microarrays and compared the results with those obtained with other inducers of differentiation, such as all-trans retinoic acid (ATRA), 1 alpha,25-dihydroxyvitamin D3 (VD3) and cotylenin A (CN-A). Many genes were up-regulated, and only a small fraction were down-regulated, upon exposure to the inducers. IPA and CN-A, but not ATRA or VD3, immediately induced the expression of mRNA for the calcium-binding protein S100P. The up-regulation of S100P was confirmed at the protein expression level. We also examined the expression of other S100 proteins, including S100A8, S100A9 and S100A12, and found that IPA preferentially up-regulated S100P at the early stages of differentiation. IPA-induced differentiation of HL-60 cells was suppressed by treatment with antisense oligonucleotides against S100P, suggesting that S100P plays an important role in cell differentiation.

• Authors: Yuki Ishii, Takashi Kasukabe, Yoshio Honma
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1745 Issue 2 Pg. 156-65 (Sep 10 2005) ISSN: 0006-3002 [Print] Netherlands
• PMID: 16129123 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Calcium-Binding Proteins
• Cytokinins
• DNA, Complementary
• Diterpenes
• Neoplasm Proteins
• S100P protein, human
• cotylenin A
• N(6)-(delta(2)-isopentenyl)adenine
• Tretinoin
• Isopentenyladenosine
• Calcitriol
• Adenine

Topics

• Adenine (analogs & derivatives, metabolism)
• Calcitriol (metabolism)
• Calcium-Binding Proteins (biosynthesis, genetics, physiology)
• Cell Differentiation (drug effects, physiology)
• Cell Line, Tumor
• Cytokinins (pharmacology)
• DNA, Complementary
• Diterpenes (metabolism)
• Gene Expression Regulation, Neoplastic (physiology)
• HL-60 Cells
• Humans
• Isopentenyladenosine
• Leukemia, Myeloid (metabolism, pathology)
• Neoplasm Proteins (biosynthesis, genetics, physiology)
• Oligonucleotide Array Sequence Analysis
• Tretinoin (metabolism)
• U937 Cells
• Up-Regulation