Abstract | BACKGROUND: MATERIALS AND METHODS: Eighteen men with HALS and 18 men with HIV but without HALS were investigated. DEXA and computed tomography scan were performed to determine total body fat and visceral adipose tissue mass. Insulin sensitivity was determined by the euglycaemic clamp technique. Plasma levels of PAI-1 and cytokines were determined. In addition, PAI-1, TNF-alpha, IL-6 and IL-8 mRNA levels in subcutaneous adipose tissue were measured by real-time reverse transcriptase polymerase chain reaction. RESULTS: HALS patients were characterized by a 3-fold increased visceral adipose tissue (P < 0.001) and reduced limb fat (P < 0.01) as compared with non-HALS patients but with no difference in total fat mass between the groups. Plasma PAI-1 was increased in HALS patients (16.7 ng mL(-1) vs. 8.2 ng mL(-1), P < 0.05). Plasma PAI-1 was positively correlated with BMI (r = 0.74, P < 0.01), plasma TNF-alpha level (r = 0.64, P < 0.01), sTNFR-I (r = 0.38, P < 0.05), and visceral fat (r = 0.67, P < 0.01). Moreover, plasma PAI-1 was negatively associated with insulin sensitivity (r = -0.57, P < 0.01) and the percentage of limb fat (r = -0.57, P < 0.01). A positive correlation was found between plasma PAI-1 and TNF-alpha mRNA level. No association was, however, found between plasma PAI-1 and PAI-1 mRNA level in adipose tissue. CONCLUSION: Plasma PAI-1 is increased in HALS patients and it is suggested that dysregulation of the TNF-system (high TNFalpha and high sTNFR1) may play a role in up-regulating PAI-1 in HALS.
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Authors | G He, O Andersen, S B Haugaard, A S Lihn, S B Pedersen, S Madsbad, B Richelsen |
Journal | European journal of clinical investigation
(Eur J Clin Invest)
Vol. 35
Issue 9
Pg. 583-90
(Sep 2005)
ISSN: 0014-2972 [Print] England |
PMID | 16128865
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Interleukin-6
- Interleukin-8
- Plasminogen Activator Inhibitor 1
- RNA, Messenger
- Receptors, Tumor Necrosis Factor
- Serine Proteinase Inhibitors
- Tumor Necrosis Factor-alpha
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Topics |
- Adipose Tissue
(metabolism)
- Adult
- Body Mass Index
- Cytokines
(analysis, blood)
- HIV-Associated Lipodystrophy Syndrome
(blood, metabolism)
- Humans
- Insulin Resistance
(physiology)
- Interleukin-6
(analysis, blood)
- Interleukin-8
(analysis, blood)
- Male
- Middle Aged
- Plasminogen Activator Inhibitor 1
(analysis, blood)
- RNA, Messenger
(analysis)
- Receptors, Tumor Necrosis Factor
(analysis, blood)
- Serine Proteinase Inhibitors
(analysis, blood)
- Tumor Necrosis Factor-alpha
(analysis)
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