T cell subset and cytokine profiles in human visceral leishmaniasis during active and asymptomatic or sub-clinical infection with Leishmania donovani.

Abstract

During an epidemiological study of visceral leishmaniasis (VL) in south-west Ethiopia, 33 VL patients, 9 treated VL patients, 14 individuals with sub-clinical infection, 34 individuals with asymptomatic infection, and 19 healthy controls were studied for T cell subsets and cytokine profiles. Negative leishmanin skin test, CD3 and CD4 lymphocytopenia, and significantly reduced numbers of memory CD4+ T cells were found in VL patients compared to treated VL patients or persons with self-limiting asymptomatic infection. The proportion of CD4+ and CD8+ T cells that produced IFN-gamma and IL-4 after stimulation with PMA (Phorbol 12-myristate 13-acetate) and ionomycin was significantly reduced in VL patients compared to sub-clinical and asymptomatic infections or healthy controls. Plasma concentrations of IFN-gamma and IL-10 were elevated in VL.

• Authors: Asrat Hailu, Debbie van Baarle, Gerlinde J Knol, Nega Berhe, Frank Miedema, Piet A Kager
• Journal: Clinical immunology (Orlando, Fla.) (Clin Immunol) Vol. 117 Issue 2 Pg. 182-91 (Nov 2005) ISSN: 1521-6616 [Print] United States
• PMID: 16125466 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Tumor Necrosis Factor Receptor Superfamily, Member 7
• Interleukin-10
• Interleukin-4
• Interferon-gamma
• Leukocyte Common Antigens

Topics

• Adolescent
• Adult
• Animals
• CD4-Positive T-Lymphocytes (metabolism)
• CD8-Positive T-Lymphocytes (metabolism)
• Child
• Cytokines (biosynthesis)
• Humans
• Interferon-gamma (blood, metabolism)
• Interleukin-10 (blood)
• Interleukin-4 (blood, metabolism)
• Leishmania donovani (immunology)
• Leishmaniasis, Visceral (immunology)
• Leukocyte Common Antigens (metabolism)
• Middle Aged
• T-Lymphocyte Subsets (metabolism)
• Tumor Necrosis Factor Receptor Superfamily, Member 7 (metabolism)