HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Photodynamic damages induced by a monocationic porphyrin derivative in a human carcinoma cell line.

Abstract
The photokilling activity of 5-(4-trimethylammoniumphenyl)-10,15,20-tris(2,4,6-trimethoxyphenyl)porphyrin (CP) was evaluated on a Hep-2 human larynx-carcinoma cell line. Cell treatment was carried out with 5 microM CP incorporated into liposomal vesicles. Under violet-blue exciting light, the red fluorescence of CP was mainly detected as a filamentous pattern characteristic of mitochondrial localization. Similar pattern was also observed using rhodamine 123 in Hep-2 cells. No dark cytotoxicity was observed using 5 microM CP concentration and long incubation time (24 h). Using Hoechst-33258 and caspase-3 immunostaining methods, cell cultures treated for 24 h with CP and exposed to light for 7.5 min (27 J/cm2) showed a great amount of apoptotic cells (40%). In contrast, necrotic cells (58%) were observed using the same drug concentration but irradiated for 15 min (54 J/cm2). The results show that CP can induce different mechanisms of cell death depending on irradiation doses in the photodynamic treatments, which makes this agent an interesting sensitizer with potential application in photodynamic tumor therapy.
AuthorsM Gabriela Alvarez, Fernando Príncipe, M Elisa Milanesio, Edgardo N Durantini, Viviana Rivarola
JournalThe international journal of biochemistry & cell biology (Int J Biochem Cell Biol) Vol. 37 Issue 12 Pg. 2504-12 (Dec 2005) ISSN: 1357-2725 [Print] Netherlands
PMID16125432 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • 5-(4-(trimethylammonium)phenyl)-10,15,20-tris(2,4,6-trimethoxyphenyl)porphyrin
  • Liposomes
  • Photosensitizing Agents
  • Porphyrins
  • Rhodamine 123
  • CASP3 protein, human
  • Caspase 3
  • Caspases
Topics
  • Apoptosis (drug effects)
  • Carcinoma (drug therapy, metabolism)
  • Caspase 3
  • Caspases (metabolism)
  • Cell Death (drug effects, radiation effects)
  • Cell Line, Tumor
  • Darkness
  • Enzyme Activation
  • Humans
  • Laryngeal Neoplasms (drug therapy, metabolism)
  • Liposomes
  • Microscopy, Fluorescence
  • Mitochondria (metabolism)
  • Necrosis (chemically induced)
  • Photochemotherapy (methods)
  • Photosensitizing Agents (metabolism, therapeutic use)
  • Porphyrins (metabolism, therapeutic use)
  • Rhodamine 123 (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: