[Effect of SiO2 on the expression of MMP-9 and TIMP-1 in human alveolar macrophages in vitro].

Abstract

OBJECTIVE:

To study the effect of SiO(2) on the expression of matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) in human alveolar macrophages (AMs) associated with the pathogenesis of silicotic fibrosis.

METHODS:

AMs were collected from a silicotic patient by bronchoalveolar lavage, and exposed to SiO(2) (50 microg/ml), and cultured in DMEM without serum for different time (2, 6, 12, 18, 24, 36 h). Immunocytochemical method was used to detect the level of expression of MMP-9 and TIMP-1 in AMs.

RESULTS:

The expression of MMP-9 in AMs exposed to silica was up-regulated, and reached the peak at 18 h [average optical density: (0.440 +/- 0.021) vs (0.390 +/- 0.011), P < 0.05]. After that, the expression reduced markedly. However, the expression of TIMP-1 of AMs were not significantly different from the control group [average optical density: (0.175 +/- 0.019) vs (0.162 +/- 0.044), P > 0.05].

CONCLUSION:

SiO(2) could induce up-expression of MMP-9 in AMs. Degradation of basement membrane by MMP-9 produced by AMs at early stage of lung injury may associate with the immigration of various cells including lung fibroblasts into the injured region.

• Authors: Xiao-bing Ma, Xiu-ling Li, Shu-xun Sun, Fang Yang
• Journal: Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases (Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi) Vol. 23 Issue 3 Pg. 203-5 (Jun 2005) ISSN: 1001-9391 [Print] China
• PMID: 16124900 (Publication Type: English Abstract, Journal Article)

Chemical References

• Tissue Inhibitor of Metalloproteinase-1
• Silicon Dioxide
• Matrix Metalloproteinase 9

Topics

• Bronchoalveolar Lavage Fluid (cytology)
• Cells, Cultured
• Humans
• Immunohistochemistry
• Macrophages, Alveolar (drug effects, metabolism)
• Male
• Matrix Metalloproteinase 9 (biosynthesis)
• Middle Aged
• Silicon Dioxide (pharmacology)
• Silicosis (pathology)
• Tissue Inhibitor of Metalloproteinase-1 (biosynthesis)