Cumulative and bolus in vitro contracture testing with 4-chloro-3-ethylphenol in malignant hyperthermia positive and negative human skeletal muscles.

Abstract

In this study we evaluated the in vitro effects of 4-chloro-3-ethylphenol (CEP) using cumulative (12.5-200 micromol/L) or bolus (75 and 100 micromol/L) administrations, on muscle specimens from malignant hyperthermia (MH) susceptible and MH nonsusceptible patients, respectively. In the cumulative CEP in vitro contracture test, contractures were significantly greater in the MH susceptible compared with the MH nonsusceptible muscles in all concentrations between 25 and 100 micromol/L. There was no overlap between the diagnostic groups at 75 micromol/L of CEP, so this test appears to be feasible for diagnosis of MH susceptibility. The two bolus tests are not diagnostically useful, as overlaps between the diagnostic groups were observed.

• Authors: Mark Ulrich Gerbershagen, Marko Fiege, Ralf Weisshorn, Kerstin Kolodzie, Jochen Schulte Am Esch, Frank Wappler
• Journal: Anesthesia and analgesia (Anesth Analg) Vol. 101 Issue 3 Pg. 710-714 (Sep 2005) ISSN: 0003-2999 [Print] United States
• PMID: 16115979 (Publication Type: Journal Article)

Chemical References

• Chlorophenols
• 4-chloro-3-ethylphenol
• Dimethyl Sulfoxide

Topics

• Adolescent
• Adult
• Aged
• Child
• Child, Preschool
• Chlorophenols (pharmacology)
• Dimethyl Sulfoxide
• Female
• Humans
• In Vitro Techniques
• Male
• Malignant Hyperthermia (diagnosis, physiopathology)
• Middle Aged
• Muscle, Skeletal (drug effects)
• Predictive Value of Tests