[Effect of nylestriol and levonorgestrel on the expression of Opg/OPGL in human osteosarcoma MG-63 cell lines].

Abstract

OBJECTIVE:

To determine the effect of nylestriol and levonorgestrel on mRNA expression of OPG/OPGL in MG-63 cell lines, and to explore whether the paracrine effect is involved in the regulation course. Methods Semi-quantitive RT-PCR was conducted to analyze mRNA expression of OPG/OPGL in MG-63 cell lines treated with nylestriol and levonorgestrel. Results Both medicines could stimulate MG-63 cell lines to express OPG, and the best action levels of the two medicines were 10(-6) mol/L. Both medicines could decrease mRNA expression levels of OPGL dramatically. In NYL 10(-10) mol/L treatment group, medium renewal has negative effect on mRNA expression of OPG. Conclusion Both the two medicines can promote mRNA expression of OPG in MG-63 cell lines, while decrease mRNA expression of OPGL. The paracrine effect of MG-63 cell lines may be involved in the regulation of mRNA expression of OPG by NYL.

• Authors: Kai-chu Yang, Er-yuan Liao, Hou-de Zhou, Xin-hua Xiao
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 29 Issue 6 Pg. 667-70 (Dec 2004) ISSN: 1672-7347 [Print] China
• PMID: 16114553 (Publication Type: Journal Article)

Chemical References

• Carrier Proteins
• Glycoproteins
• Membrane Glycoproteins
• Osteoprotegerin
• RANK Ligand
• RNA, Messenger
• Receptor Activator of Nuclear Factor-kappa B
• Receptors, Cytoplasmic and Nuclear
• Receptors, Tumor Necrosis Factor
• TNFRSF11A protein, human
• TNFRSF11B protein, human
• TNFSF11 protein, human
• Levonorgestrel
• nylestriol
• Quinestrol

Topics

• Carrier Proteins (biosynthesis, genetics)
• Female
• Glycoproteins (biosynthesis, genetics)
• Humans
• Levonorgestrel (pharmacology)
• Membrane Glycoproteins (biosynthesis, genetics)
• Osteoprotegerin
• Osteosarcoma (pathology)
• Quinestrol (analogs & derivatives, pharmacology)
• RANK Ligand
• RNA, Messenger (biosynthesis, genetics)
• Receptor Activator of Nuclear Factor-kappa B
• Receptors, Cytoplasmic and Nuclear (biosynthesis, genetics)
• Receptors, Tumor Necrosis Factor (biosynthesis, genetics)
• Tumor Cells, Cultured