Abstract | BACKGROUND: In Japan, postoperative relapse occurs within five years in 9.2 to 16% of patients whose breast cancers have not metastasized to lymph nodes at the time of initial surgery(node-negative, n0). Attempts to find molecular markers able to classify n0 breast cancers in terms of postoperative prognosis have not been successful. METHODS: To identify molecular indicators of prognosis for this type of cancer, we used a cDNA microarray consisting of 25,344 human genes to investigate expression profiles of 12 primary breast cancers from patients whose tumors recurred within five years after surgery(5Y-R) and 12 from patients who survived disease-free for more than five years (5Y-F). RESULTS: Sets of genes characterizing each group in terms of expression patterns in the tumors were selected by Mann-Whitney and random-permutation tests: these panels included 21 genes expressed highly in 5Y-R tumors than in 5Y-F tumors, and 37 with higher expression in the 5Y-F group than in the 5Y-R group. CONCLUSIONS: We established a scoring system to prediction of postoperative prognosis which was 100% accurate as to the actual clinical outcomes of the 24 cases and therefore might be useful for predicting prognosis of n0 breast cancers in a clinical setting. The prognostic score system clearly separated the two groups without any overlap, and accurately predicted prognosis in 6 additional cases. Moreover, the extensive list of tumor-related genes identified in these experiments provides valuable information about progression of breast cancer and suggests potential target molecules for therapy of n0 breast cancers.
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Authors | Koji Tsumagari, Kazuo Chijiiwa, Hisaki Nagai, Masujiro Makita, Fujio Kasumi, Futoshi Akiyama, Goi Sakamoto, Yoshio Miki |
Journal | Breast cancer (Tokyo, Japan)
(Breast Cancer)
Vol. 12
Issue 3
Pg. 166-77
( 2005)
ISSN: 1340-6868 [Print] Japan |
PMID | 16110286
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Breast Neoplasms
(genetics, mortality, pathology)
- Carcinoma, Ductal, Breast
(genetics, mortality, pathology)
- Disease-Free Survival
- Female
- Gene Expression Profiling
(methods)
- Health Status Indicators
- Humans
- Lymph Nodes
(pathology)
- Lymphatic Metastasis
- Mastectomy
- Middle Aged
- Neoplasm Recurrence, Local
(genetics, mortality, pathology)
- Neoplasm Staging
- Oligonucleotide Array Sequence Analysis
- Postoperative Period
- Predictive Value of Tests
- Prognosis
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