Abstract | BACKGROUND: METHODS: A double-antibody ELISA for immunoreactive aCAP (ir-aCAP) was developed, and ir-aCAP was determined in the serum of patients with acute pancreatitis and characterized using gel filtration. RESULTS: Serum from healthy individuals contained minimal levels of ir-aCAP, while serum from 25 patients with acute pancreatitis contained between 0.6 and 158 nmol/l. Gel filtration of serum samples from patients with acute pancreatitis showed the presence of two peaks of ir-aCAP, one corresponding to the molecular size of the free active enzyme and one corresponding to cross-reactive proCAP. When pure aCAP was mixed with serum in vitro, all ir-aCAP was recovered as one peak of free enzyme. No immunoreactivity disappeared or changed in molecular size, as might be expected if the active enzyme was bound to an inhibitor. CONCLUSION:
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Authors | Anders Borgström, Sara Regnér |
Journal | Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]
(Pancreatology)
Vol. 5
Issue 6
Pg. 530-6
( 2005)
ISSN: 1424-3903 [Print] Switzerland |
PMID | 16110251
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2005 S. Karger AG, Basel and IAP. |
Chemical References |
- CPB1 protein, human
- Peptides
- carboxypeptidase B activation peptide
- C-Reactive Protein
- Carboxypeptidase B
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Topics |
- Acute Disease
- C-Reactive Protein
(analysis)
- Carboxypeptidase B
(blood)
- Chromatography, Gel
- Cross Reactions
- Enzyme Activation
- Enzyme-Linked Immunosorbent Assay
(methods)
- Pancreatitis
(enzymology)
- Peptides
(blood)
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