Surface properties of sulfur- and ether-linked phosphonolipids with and without purified hydrophobic lung surfactant proteins.

Abstract

Two novel C16:0 sulfur-linked phosphonolipids (S-lipid and SO(2)-lipid) and two ether-linked phosphonolipids (C16:0 DEPN-8 and C16:1 UnDEPN-8) were studied for surface behavior alone and in mixtures with purified bovine lung surfactant proteins (SP)-B and/or SP-C. Synthetic C16:0 phosphonolipids all had improved adsorption and film respreading compared to dipalmitoyl phosphatidylcholine, and SO(2)-lipid and DEPN-8 reached maximum surface pressures of 72mN/m (minimum surface tensions of <1mN/m) in compressed films on the Wilhelmy balance (23 degrees C). Dispersions of DEPN-8 (0.5mg/ml) and SO(2)-lipid (2.5mg/ml) also reached minimum surface tensions of <1mN/m on a pulsating bubble surfactometer (37 degrees C, 20cycles/min, 50% area compression). Synthetic lung surfactants containing DEPN-8 or SO(2)-lipid+0.75% SP-B+0.75% SP-C had dynamic surface activity on the bubble equal to that of calf lung surfactant extract (CLSE). Surfactants containing DEPN-8 or SO(2)-lipid plus 1.5% SP-B also had very high surface activity, but less than when both apoproteins were present together. Adding 10wt.% of UnDEPN-8 to synthetic lung surfactants did not improve dynamic surface activity. Surfactants containing DEPN-8 or SO(2)-lipid plus 0.75% SP-B/0.75% SP-C were chemically and biophysically resistant to phospholipase A(2) (PLA(2)), while CLSE was severely inhibited by PLA(2). The high activity and inhibition resistance of synthetic surfactants containing DEPN-8 or SO(2)-lipid plus SP-B/SP-C are promising for future applications in treating surfactant dysfunction in inflammatory lung injury.

• Authors: Yusuo Chang, Zhengdong Wang, Adrian L Schwan, Zhongyi Wang, Bruce A Holm, John E Baatz, Robert H Notter
• Journal: Chemistry and physics of lipids (Chem Phys Lipids) Vol. 137 Issue 1-2 Pg. 77-93 (Oct 2005) ISSN: 0009-3084 [Print] Ireland
• PMID: 16109391 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Ethers
• Phospholipids
• Pulmonary Surfactant-Associated Protein B
• Pulmonary Surfactant-Associated Protein C
• Sulfones
• phosphonolipids
• Phospholipases A

Topics

• Adsorption
• Animals
• Cattle
• Ethers (chemistry)
• Hydrophobic and Hydrophilic Interactions
• Phospholipases A (chemistry)
• Phospholipids (chemistry)
• Pulmonary Surfactant-Associated Protein B (chemistry)
• Pulmonary Surfactant-Associated Protein C (chemistry)
• Sulfones (chemistry)
• Surface Tension
• Thermodynamics