Abstract |
Regulated interactions between microtubules (MTs) and the cell cortex control MT dynamics and position the mitotic spindle. In eukaryotic cells, the adenomatous polyposis coli/Kar9p and dynein/ dynactin pathways are involved in guiding MT plus ends and MT sliding along the cortex, respectively. Here we identify Bud14p as a novel cortical activator of the dynein/ dynactin complex in budding yeast. Bud14p accumulates at sites of polarized growth and the mother-bud neck during cytokinesis. The localization to bud and shmoo tips requires an intact actin cytoskeleton and the kelch-domain-containing proteins Kel1p and Kel2p. While cells lacking Bud14p function fail to stabilize the pre-anaphase spindle at the mother-bud neck, overexpression of Bud14p is toxic and leads to elongated astral MTs and increased dynein-dependent sliding along the cell cortex. Bud14p physically interacts with the type-I phosphatase Glc7p, and localizes Glc7p to the bud cortex. Importantly, the formation of Bud14p-Glc7p complexes is necessary to regulate MT dynamics at the cortex. Taken together, our results suggest that Bud14p functions as a regulatory subunit of the Glc7p type-I phosphatase to stabilize MT interactions specifically at sites of polarized growth.
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Authors | Michèle Knaus, Elisabetta Cameroni, Ivo Pedruzzi, Kelly Tatchell, Claudio De Virgilio, Matthias Peter |
Journal | The EMBO journal
(EMBO J)
Vol. 24
Issue 17
Pg. 3000-11
(Sep 07 2005)
ISSN: 0261-4189 [Print] England |
PMID | 16107882
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adaptor Proteins, Signal Transducing
- Bud14 protein, S cerevisiae
- Carrier Proteins
- Dynactin Complex
- KEL1 protein, S cerevisiae
- KEL2 protein, S cerevisiae
- Microtubule-Associated Proteins
- Saccharomyces cerevisiae Proteins
- GLC7 protein, S cerevisiae
- Phosphoprotein Phosphatases
- Protein Phosphatase 1
- Dyneins
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Topics |
- Actin Cytoskeleton
(metabolism)
- Adaptor Proteins, Signal Transducing
- Amino Acid Motifs
- Carrier Proteins
(metabolism)
- Conserved Sequence
- Cytokinesis
- Dynactin Complex
- Dyneins
(metabolism)
- Microtubule-Associated Proteins
(metabolism)
- Microtubules
(metabolism)
- Mitosis
- Mutation
- Phosphoprotein Phosphatases
(genetics, metabolism)
- Protein Binding
- Protein Phosphatase 1
- Saccharomyces cerevisiae
(genetics, metabolism)
- Saccharomyces cerevisiae Proteins
(genetics, metabolism)
- Spindle Apparatus
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