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Epstein-Barr virus infection in sarcomatoid renal cell carcinoma tissues.

AbstractOBJECTIVE:
To determine whether Epstein-Barr virus (EBV) infection is related to renal cell carcinoma (RCC) tissues.
MATERIALS AND METHODS:
We investigated EBV infection and its genotypes in 73 cases of different types of RCC and 18 of non-neoplastic kidney. EBV infection and its genotypes were determined by EBV-encoded RNAs in situ hybridization (EBER-ISH) and polymerase chain reactions for EBV-encoded nuclear antigen 1 (EBNA-1) and EBNA-3C. The immunophenotype and EBV status of the EBV-infected cells were examined by double-labelling of EBER-ISH and/or immunohistochemistry for lymphoid cell markers, EBV proteins, and CD21.
RESULTS:
EBER-ISH signals were detected in five of 73 RCC tissues (6.8%), but in none of 18 non-neoplastic kidneys. Interestingly, EBER-ISH was positive only in five of the 10 sarcomatoid RCCs, and of these, four also showed amplification of EBNA-1. EBV was located exclusively in the tumour-infiltrating B lymphocytes of sarcomatoid RCCs. The genotype of EBV was determined as type 1. A few EBV-infected B cells expressed BZLF1 (an EBV immediate-early gene product) while none expressed EBNA-2 or latent membrane protein 1. This indicates that the B cells are of EBV latency type I, often replicating EBV. EBV infection did not affect the survival rates of patients with sarcomatoid RCC (P = 0.635, Kaplan-Meier analysis, log-rank test).
CONCLUSION:
EBV is present only in tumour-infiltrating B lymphocytes of sarcomatoid RCCs. The present study suggests that sarcomatoid RCC modulates a function of EBV-specific T cells controlling EBV replication, or stimulates differentiation of memory B cells into plasma cells.
AuthorsKwang Hee Kim, Eun Mee Han, Eung Seok Lee, Hong Seok Park, Insun Kim, Young-Sik Kim
JournalBJU international (BJU Int) Vol. 96 Issue 4 Pg. 547-52 (Sep 2005) ISSN: 1464-4096 [Print] England
PMID16104908 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Viral
  • Biomarkers
  • EBNA-3C, epstein-barr virus
  • Epstein-Barr Virus Nuclear Antigens
  • RNA, Viral
  • Receptors, Complement 3d
  • EBV-encoded nuclear antigen 1
Topics
  • Adult
  • Aged
  • Antigens, Viral (genetics)
  • B-Lymphocytes (virology)
  • Biomarkers (analysis)
  • Carcinoma, Renal Cell (immunology, pathology, virology)
  • Epstein-Barr Virus Infections (complications, immunology)
  • Epstein-Barr Virus Nuclear Antigens (genetics)
  • Female
  • Genotype
  • Herpesvirus 4, Human (genetics)
  • Humans
  • Immunohistochemistry (methods)
  • In Situ Hybridization
  • Kidney Neoplasms (immunology, pathology, virology)
  • Lymphocytes, Tumor-Infiltrating (virology)
  • Male
  • Middle Aged
  • Mixed Tumor, Malignant (immunology, pathology, virology)
  • Polymerase Chain Reaction (methods)
  • RNA, Viral (analysis)
  • Receptors, Complement 3d (analysis)
  • Sarcoma (immunology, virology)
  • Virus Latency

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