Abstract | OBJECTIVE: MATERIALS AND METHODS: We investigated EBV infection and its genotypes in 73 cases of different types of RCC and 18 of non-neoplastic kidney. EBV infection and its genotypes were determined by EBV-encoded RNAs in situ hybridization (EBER-ISH) and polymerase chain reactions for EBV-encoded nuclear antigen 1 (EBNA-1) and EBNA-3C. The immunophenotype and EBV status of the EBV-infected cells were examined by double-labelling of EBER-ISH and/or immunohistochemistry for lymphoid cell markers, EBV proteins, and CD21. RESULTS: EBER-ISH signals were detected in five of 73 RCC tissues (6.8%), but in none of 18 non-neoplastic kidneys. Interestingly, EBER-ISH was positive only in five of the 10 sarcomatoid RCCs, and of these, four also showed amplification of EBNA-1. EBV was located exclusively in the tumour-infiltrating B lymphocytes of sarcomatoid RCCs. The genotype of EBV was determined as type 1. A few EBV-infected B cells expressed BZLF1 (an EBV immediate-early gene product) while none expressed EBNA-2 or latent membrane protein 1. This indicates that the B cells are of EBV latency type I, often replicating EBV. EBV infection did not affect the survival rates of patients with sarcomatoid RCC (P = 0.635, Kaplan-Meier analysis, log-rank test). CONCLUSION: EBV is present only in tumour-infiltrating B lymphocytes of sarcomatoid RCCs. The present study suggests that sarcomatoid RCC modulates a function of EBV-specific T cells controlling EBV replication, or stimulates differentiation of memory B cells into plasma cells.
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Authors | Kwang Hee Kim, Eun Mee Han, Eung Seok Lee, Hong Seok Park, Insun Kim, Young-Sik Kim |
Journal | BJU international
(BJU Int)
Vol. 96
Issue 4
Pg. 547-52
(Sep 2005)
ISSN: 1464-4096 [Print] England |
PMID | 16104908
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Viral
- Biomarkers
- EBNA-3C, epstein-barr virus
- Epstein-Barr Virus Nuclear Antigens
- RNA, Viral
- Receptors, Complement 3d
- EBV-encoded nuclear antigen 1
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Topics |
- Adult
- Aged
- Antigens, Viral
(genetics)
- B-Lymphocytes
(virology)
- Biomarkers
(analysis)
- Carcinoma, Renal Cell
(immunology, pathology, virology)
- Epstein-Barr Virus Infections
(complications, immunology)
- Epstein-Barr Virus Nuclear Antigens
(genetics)
- Female
- Genotype
- Herpesvirus 4, Human
(genetics)
- Humans
- Immunohistochemistry
(methods)
- In Situ Hybridization
- Kidney Neoplasms
(immunology, pathology, virology)
- Lymphocytes, Tumor-Infiltrating
(virology)
- Male
- Middle Aged
- Mixed Tumor, Malignant
(immunology, pathology, virology)
- Polymerase Chain Reaction
(methods)
- RNA, Viral
(analysis)
- Receptors, Complement 3d
(analysis)
- Sarcoma
(immunology, virology)
- Virus Latency
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