Intracellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein: absence of nucleolar accumulation during infection and after expression as a recombinant protein in vero cells.

Abstract

The nucleocapsid (N) protein of several members within the order Nidovirales localizes to the nucleolus during infection and after transfection of cells with N genes. However, confocal microscopy of N protein localization in Vero cells infected with the severe acute respiratory syndrome coronavirus (SARS-CoV) or transfected with the SARS-CoV N gene failed to show the presence of N in the nucleoplasm or nucleolus. Amino acids 369 to 389, which contain putative nuclear localization signal (NLS) and nucleolar localization signal motifs, failed to restore nuclear localization to an NLS-minus mutant Rev protein. These data indicate that nuclear localization is not a conserved property among all nidoviruses.

• Authors: Raymond R R Rowland, Vinita Chauhan, Ying Fang, Andrew Pekosz, Maureen Kerrigan, Miriam D Burton
• Journal: Journal of virology (J Virol) Vol. 79 Issue 17 Pg. 11507-12 (Sep 2005) ISSN: 0022-538X [Print] United States
• PMID: 16103202 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Coronavirus Nucleocapsid Proteins
• Nuclear Localization Signals
• Nucleocapsid Proteins
• Recombinant Proteins

Topics

• Amino Acid Motifs (genetics)
• Amino Acid Sequence
• Animals
• Chlorocebus aethiops
• Coronavirus Nucleocapsid Proteins
• Molecular Sequence Data
• Nuclear Localization Signals (metabolism)
• Nucleocapsid Proteins (genetics, metabolism)
• Recombinant Proteins (metabolism)
• Severe acute respiratory syndrome-related coronavirus (physiology)
• Vero Cells (metabolism)