[Synthesis of purpure-18-imide and study on its interaction with bovine serum albumins by fluorescence].

Abstract

Purpure-18-imide was synthesized based on the porphyrin's special affinity for cancer cells and the antitumor activity of pyrrolidine compound. The binding reaction between purpure-18-imide and bovine serum albumins (BSA) in aqueous was studied by fluorescence and UV-Vis absorption spectra. The research results indicated that the combination reaction of them was a single static quenching process. In aqueous, purpure-18-imide strongly bound BSA with molar ratio of 1:1. The binding constant K0 was 5.386 x 10(5) L x mol(-1). The shortest binding distance (r = 3.54 nm) and energy transfer efficiencies (E = 0.26) between donor (BSA) and acceptor (purpure-18-imide) were obtained by Forster's nonradiative energy transfer mechanism.

• Authors: Jin-jun Wang, Yong-ming Liu, Li Li
• Journal: Guang pu xue yu guang pu fen xi = Guang pu (Guang Pu Xue Yu Guang Pu Fen Xi) Vol. 25 Issue 4 Pg. 594-7 (Apr 2005) ISSN: 1000-0593 [Print] China
• PMID: 16097695 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Imides
• Porphyrins
• purpure-18-imide
• purpurin 18
• Serum Albumin, Bovine

Topics

• Algorithms
• Animals
• Cattle
• Energy Transfer
• Fluorescence
• Imides (chemical synthesis, chemistry, metabolism)
• Kinetics
• Models, Chemical
• Molecular Structure
• Porphyrins (chemical synthesis, chemistry)
• Protein Binding
• Serum Albumin, Bovine (chemistry, metabolism)
• Spectrometry, Fluorescence
• Spectrophotometry
• Spectrophotometry, Ultraviolet