Abstract | OBJECTIVE: The goal of this study was to determine the utility of adeno-associated virus (AAV) vectors for anti-angiogenic gene therapy in a mouse model of collagen-induced arthritis (CIA). METHODS: RESULTS: AAV vectors were capable of efficient gene transfer into chondrocytes and synovial cells, and the extent of synovial hyperplasia and other parameters of arthritis were significantly reduced in the knee joints injected with AAV-Ang/GFP compared with the joints treated with either AAV-GFP/NeoR or phosphate-buffered solution (PBS). Reduction in the number of vessels was confirmed in AAV-Ang/GFP-treated joints. CONCLUSION:
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Authors | H Takahashi, K Kato, K Miyake, Y Hirai, S Yoshino, T Shimada |
Journal | Clinical and experimental rheumatology
(Clin Exp Rheumatol)
2005 Jul-Aug
Vol. 23
Issue 4
Pg. 455-61
ISSN: 0392-856X [Print] Italy |
PMID | 16095112
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Angiogenesis Inhibitors
- RNA, Messenger
- Angiostatins
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Topics |
- Angiogenesis Inhibitors
(genetics)
- Angiostatins
(genetics)
- Animals
- Arthritis, Experimental
(pathology, prevention & control, therapy)
- Chondrocytes
(metabolism, pathology)
- Dependovirus
(genetics)
- Female
- Gene Expression
- Genetic Therapy
- Genetic Vectors
- HeLa Cells
- Humans
- Joints
(metabolism, pathology)
- Mice
- Mice, Inbred DBA
- RNA, Messenger
(analysis)
- Reverse Transcriptase Polymerase Chain Reaction
- Synovial Membrane
(blood supply, pathology)
- Transduction, Genetic
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