Mutation of DNA polymerase beta in esophageal carcinoma of different regions.

Abstract

AIM:

To observe the variation of DNA polymerase beta (polbeta) in esophageal carcinoma.

METHODS:

Thirty specimens containing adjacent normal epithelial tissues were collected from patients in Linzhou region (a high risk area for esophageal squamous carcinoma) and 25 specimens were from a non-high risk area. Total RNA was extracted from the samples and reverse transcription polymerase chain reaction (RT-PCR) was performed. PCR products were cloned and sequenced to investigate the polbeta gene with DNASIS and OMIGA. Statistical significance was evaluated using the chi(2) test.

RESULTS:

High-incidence area group: polbeta gene variation was detected in 13 of 30 esophageal carcinoma tissue specimens, and only one variation was found in 30 corresponding adjacent normal tissue specimens. Non high-incidence area group: polbeta gene variation was detected in 5 of 25 esophageal carcinoma tissue specimens, and no variation was found in 25 corresponding adjacent normal tissue specimens. The incidence of polbeta gene variation observed in the high-incidence area group was significantly higher than in the non-high incidence area group. Two mutation hot spots (454-466 and 648-670 nt) and a 58 bp deletion (177-234 nt) were found.

CONCLUSION:

Variations of polbeta perform different functions between the high-incidence areas and the other areas, and may play a more important role in the high-incidence areas.

• Authors: Guo-Qiang Zhao, Tao Wang, Qin Zhao, Hong-Yan Yang, Xiao-Hui Tan, Zi-Ming Dong
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 11 Issue 30 Pg. 4618-22 (Aug 14 2005) ISSN: 1007-9327 [Print] United States
• PMID: 16094698 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Neoplasm
• DNA Polymerase beta

Topics

• Base Sequence
• Carcinoma, Squamous Cell (enzymology, genetics)
• China
• DNA Polymerase beta (genetics)
• DNA, Neoplasm (genetics)
• Esophageal Neoplasms (enzymology, genetics)
• Humans
• Molecular Sequence Data
• Mutation
• Sequence Homology, Nucleic Acid