Abstract | INTRODUCTION: METHOD: RESULTS: The procedure is rapid and reproducible, giving derivatives with excellent chromatographic properties. Using this procedure, it has now been shown that beta-phenylethylamine (PEA), N-methylphenylethylamine (N-methylPEA) and N-propargylphenylethylamine (N-propargylPEA) are formed from MPPE during incubation of this drug with human liver microsomes. Levels of all three metabolites were shown to increase with increasing time of incubation with the microsomes. DISCUSSION: Extractive derivatization with PFBSC followed by electron-capture gas chromatography represents an efficient means of separating and quantitating the metabolites of MPPE, a novel neuroprotective agent.
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Authors | Kay Rittenbach, B Duff Sloley, Lei Ling, Ronald T Coutts, Jackie Shan, Glen B Baker |
Journal | Journal of pharmacological and toxicological methods
(J Pharmacol Toxicol Methods)
2005 Nov-Dec
Vol. 52
Issue 3
Pg. 373-8
ISSN: 1056-8719 [Print] United States |
PMID | 16087356
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- N-Methyl,N-propargyphenylethylamine
- Neuroprotective Agents
- Phenethylamines
- Sulfones
- N-methylphenethylamine
- Selegiline
- phenethylamine
- Methamphetamine
- pentafluorobenzenesulfonyl chloride
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Topics |
- Gas Chromatography-Mass Spectrometry
(methods)
- Humans
- In Vitro Techniques
- Methamphetamine
(analogs & derivatives, analysis, metabolism)
- Microsomes, Liver
(metabolism)
- Neuroprotective Agents
(analysis, metabolism)
- Phenethylamines
(analysis, metabolism)
- Selegiline
(analogs & derivatives, analysis, metabolism)
- Sulfones
- Time Factors
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