Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli.

Abstract

A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amylose affinity column. The purity of product was greater than 98% and yielded an average of 5.5 g kg(-1) dry cells. Recombinant allophycocyanin significantly inhibited H22 hepatoma (p ( 0.01) in mice with inhibition rates ranging from 36% to 62% with doses from 6.25 to 50 mg kg(-1) d(-1).

• Authors: Baosheng Ge, Zhihong Tang, Liping Lin, Yuhong Ren, Yu Yang, Song Qin
• Journal: Biotechnology letters (Biotechnol Lett) Vol. 27 Issue 11 Pg. 783-7 (Jun 2005) ISSN: 0141-5492 [Print] Netherlands
• PMID: 16086260 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Recombinant Proteins
• allophycocyanin
• Phycocyanin

Topics

• Animals
• Bioreactors (microbiology)
• Carcinoma, Hepatocellular (pathology, prevention & control)
• Cell Line, Tumor
• Chromatography, High Pressure Liquid
• Dose-Response Relationship, Drug
• Electrophoresis, Polyacrylamide Gel
• Escherichia coli (genetics, growth & development, metabolism)
• Female
• Fermentation
• Gene Expression
• Liver Neoplasms, Experimental (pathology, prevention & control)
• Mice
• Neoplasm Transplantation
• Phycocyanin (genetics, isolation & purification, pharmacology)
• Recombinant Proteins (biosynthesis, isolation & purification, pharmacology)