Abstract | OBJECTIVE: DESIGN: Case-control study. SETTING: In vitro fertilization program at the Showa University School of Medicine. PATIENT(S): INTERVENTION(S): Immunohistochemical staining for STS. Measurement of STS mRNA expression by real-time polymerase chain reaction (PCR). MAIN OUTCOME MEASURE(S): Expression of STS mRNA and localization of STS. RESULT(S):
Steroid sulfatase was localized in the cytoplasm of the cumulus cells, and expression of STS mRNA was observed. The expression level of STS mRNA from patients with endometriosis was significantly higher (11.8-fold) than that of patients without endometriosis. CONCLUSION(S): These results suggest a local steroidal regulation mechanism in cumulus cells. Although the physiological role of STS in cumulus cells remains unclear, STS may be involved in the quality of eggs in patients with endometriosis.
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Authors | Atsushi Yanaihara, Yukiko Otsuka, Shinji Iwasaki, Takashi Okai, Takumi Yanaihara |
Journal | Fertility and sterility
(Fertil Steril)
Vol. 84
Issue 2
Pg. 464-7
(Aug 2005)
ISSN: 1556-5653 [Electronic] United States |
PMID | 16084891
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- RNA, Messenger
- Steryl-Sulfatase
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Topics |
- Adult
- Case-Control Studies
- Endometriosis
(enzymology, genetics, pathology)
- Female
- Gene Expression Regulation, Enzymologic
(genetics, physiology)
- Humans
- Oocytes
(enzymology, pathology)
- RNA, Messenger
(biosynthesis)
- Steryl-Sulfatase
(biosynthesis, genetics, physiology)
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