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Compensatory recovery of liver mass by Akt-mediated hepatocellular hypertrophy in liver-specific STAT3-deficient mice.

AbstractBACKGROUND/AIMS:
Liver regeneration following hepatectomy is complicated and involves a variety of interacting factors. The present study was designed to study the roles of proliferation and hypertrophy of hepatocytes in liver regeneration following hepatectomy in liver-specific STAT3-knockout (LS3-KO) mice lacking mitogenic activity.
METHODS:
Partial hepatectomy was performed in LS3-KO and control mice. Liver regeneration was estimated by the liver weight, cell proliferation and cell size, and the related cellular signals were analyzed.
RESULTS:
Proliferation of hepatocytes following PH was markedly suppressed in LS3-KO mice with reduced cyclinD1 transcript. However, liver mass recovered sufficiently following PH in LS3-KO mice almost equal to that of control mice. Analysis of hepatocellular growth revealed that cell size following hepatectomy was significantly larger in LS3-KO mice than in control mice. Hepatectomy induced immediate but transient phosphorylation of Akt, p70S6K, mTOR and GSK-3beta in LS3-KO mice much more than in control mice. Additionally, adenoviral transfection of dominant negative mutant of Akt to control and LS3-KO mice led to insufficient liver regeneration following hepatectomy.
CONCLUSIONS:
PI3-K/Akt-mediated responsive hepatocellular hypertrophy may be essential for liver regeneration following hepatectomy and sufficiently compensated liver regeneration even in STAT3-deficient liver, in which cell proliferation is impaired.
AuthorsSanae Haga, Wataru Ogawa, Hiroshi Inoue, Keita Terui, Tetsuya Ogino, Rumi Igarashi, Kiyoshi Takeda, Shizuo Akira, Shin Enosawa, Hiroyuki Furukawa, Satoru Todo, Michitaka Ozaki
JournalJournal of hepatology (J Hepatol) Vol. 43 Issue 5 Pg. 799-807 (Nov 2005) ISSN: 0168-8278 [Print] Netherlands
PMID16083985 (Publication Type: Journal Article)
Chemical References
  • STAT3 Transcription Factor
  • Serum Albumin
  • Stat3 protein, mouse
  • Protein Kinases
  • mTOR protein, mouse
  • Glycogen Synthase Kinase 3 beta
  • Gsk3b protein, mouse
  • Proto-Oncogene Proteins c-akt
  • Ribosomal Protein S6 Kinases, 70-kDa
  • TOR Serine-Threonine Kinases
  • Glycogen Synthase Kinase 3
Topics
  • Animals
  • Apoptosis (physiology)
  • Cell Proliferation
  • Cell Size
  • Glycogen Synthase Kinase 3 (metabolism)
  • Glycogen Synthase Kinase 3 beta
  • Hepatectomy
  • Hepatocytes (cytology, physiology)
  • Hypertrophy
  • Liver (anatomy & histology, pathology)
  • Liver Regeneration
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Organ Size
  • Phosphatidylinositol 3-Kinases (metabolism)
  • Protein Kinases (metabolism)
  • Proto-Oncogene Proteins c-akt (metabolism)
  • Ribosomal Protein S6 Kinases, 70-kDa (metabolism)
  • STAT3 Transcription Factor (genetics, metabolism)
  • Serum Albumin (metabolism)
  • TOR Serine-Threonine Kinases

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