Analysis and frequency of bovine lymphocyte antigen (BoLA-DRB3) alleles in Iranian Holstein cattle.

Abstract

The bovine lymphocyte antigen (BoLA-DRB3) gene encodes cell surface glycoproteins that initiate immune response by presenting processed antigenic peptides to CD4 T helper cells. DRB3 is the most polymorphic bovine MHC class II gene which encodes the peptide-binding groove. DRB3 gene has been extensively evaluated as a candidate marker for association with various bovine diseases and immunological traits. This study describes genetic variability in the BoLA-DRB3 in Iranian Holstein cattle. This is the first study of the DNA polymorphism of the BoLA-DRB3 gene in Iranian Holstein cattle. Hemi-nested PCR-RFLP method is used for identification the frequency of BoLA-DRB3 alleles. The BoLA-DRB3 locus is highly polymorphic in the studied herd (26 alleles). Almost 67% of the alleles were accounted for four alleles (BoLA-DRB3.2*8, *24, *11 and *16) in Iranian Holstein cattle. The DRB3.2*8 allele frequency (26.6%) was higher than the others. The frequencies of the DRB3.2*54, *37, *36, *28, *25, *14, *13, *10, *1 alleles were lower than 1%. Significant distinctions have been found between Iranian Holstein cattle and other cattle breeds studied. In Iranian Holstein cattle the alleles (BoLA-DRB3.2*22, *2 and *16) associated with a lower risk of cystic ovarian disease in Holstein cattle are found. The alleles associated with the resistance to mastitis and to bovine leukemia virus infection BoLA-DRB3.2*11 and *23 are detected with the frequencies 10.4% and 4.4%, respectively. Thus in the Iranian Holstein cows studied are found alleles which are associated with resistance to various diseases. The method of DNA-typing of animals can be used in agricultural practice for BoLA-DRB3 allele genotyping of cattle in order to reduce spreading of alleles providing susceptibility to mastitis or leukemia in cattle herds.

• Authors: M R Nassiry, F Eftekhar Shahroodi, J Mosafer, A Mohammadi, E Manshad, S Ghazanfari, M R Mohammad Abadi, G E Sulimova
• Journal: Genetika (Genetika) Vol. 41 Issue 6 Pg. 817-22 (Jun 2005) ISSN: 0016-6758 [Print] Russia (Federation)
• PMID: 16080607 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BoLA-DRB3 antigen
• Histocompatibility Antigens Class II

Topics

• Animals
• Cattle
• Female
• Gene Frequency (genetics, immunology)
• Histocompatibility Antigens Class II (genetics, immunology)
• Iran
• Leukemia (genetics, immunology, veterinary)
• Male
• Mastitis, Bovine (genetics, immunology)