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Xanthoangelol, a major chalcone constituent of Angelica keiskei, induces apoptosis in neuroblastoma and leukemia cells.

Abstract
Xanthoangelol, a major chalcone constituent of the stem exudates of Angelica keiskei, was evaluated for cell toxicity and apoptosis-inducing activity in human neuroblastoma (IMR-32) and leukemia (Jurkat) cells. Xanthoangelol concentration-dependently reduced the survival rates of both cell lines as revealed by the trypan blue exclusion test. Early apoptosis induced by 4 h incubation with xanthoangelol was detected using flow cytometry after double-staining with annexin V and propidium iodide (PI). Western blot analysis showed that xanthoangelol markedly reduced the level of precursor caspase-3 and increased the level of cleaved caspase-3, but Bax and Bcl-2 proteins were not affected. These results suggest that xanthoangelol induces apoptotic cell death by activatation of caspase-3 in neuroblastoma and leukemia cells through a mechanism that does not involve Bax/Bcl-2 signal transduction. Therefore, xanthoangelol may be applicable as an effective drug for treatment of neuroblastoma and leukemia.
AuthorsKeiichi Tabata, Kou Motani, Noriya Takayanagi, Reiko Nishimura, Satoru Asami, Yumiko Kimura, Motohiko Ukiya, Daisuke Hasegawa, Toshihiro Akihisa, Takashi Suzuki
JournalBiological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 28 Issue 8 Pg. 1404-7 (Aug 2005) ISSN: 0918-6158 [Print] Japan
PMID16079483 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Proto-Oncogene Proteins c-bcl-2
  • Chalcone
  • xanthoangelol
  • CASP3 protein, human
  • Caspase 3
  • Caspases
Topics
  • Angelica (chemistry)
  • Apoptosis (drug effects)
  • Caspase 3
  • Caspases (metabolism)
  • Chalcone (analogs & derivatives, isolation & purification, pharmacology)
  • Enzyme Activation
  • Humans
  • Leukemia (pathology)
  • Neuroblastoma (pathology)
  • Proto-Oncogene Proteins c-bcl-2 (metabolism)
  • Tumor Cells, Cultured

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