Cytotoxic effect of 7beta-hydroxycholesterol on human NCI-H460 lung cancer cells.

Abstract

The cytotoxic activity of 7beta-hydroxycholesterol (7beta-OHC) was evaluated on human NCI-H460 lung cancer cells. 7beta-OHC decreased clonogenic survival of NCI-H460 in a dose dependent pattern. 7beta-OHC induced apoptosis in NCI-H460, with the characteristic features like increase in sub-G(1) hypodiploid (apoptotic) cells, and apoptotic body formation, as evidenced by flow cytometry and fluorescence microscope, respectively. Apoptosis was also associated with loss of mitochondrial transmembrane potential, and the activation of caspases 9 and 3. 7beta-OHC resulted in generation of reactive oxygen species (ROS) during apoptosis. On the whole, the results indicated that 7beta-OHC inhibited the proliferation of NCI-H460 cells through apoptosis via caspase activation.

• Authors: Kyoung Ah Kang, Sungwook Chae, Kyoung Hwa Lee, Moon Taek Park, Su Jae Lee, Yun Sil Lee, Jin Won Hyun
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 28 Issue 8 Pg. 1377-80 (Aug 2005) ISSN: 0918-6158 [Print] Japan
• PMID: 16079477 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hydroxycholesterols
• Reactive Oxygen Species
• cholest-5-en-3 beta,7 alpha-diol

Topics

• Carcinoma, Non-Small-Cell Lung (pathology)
• Cell Line, Tumor
• Flow Cytometry
• Humans
• Hydroxycholesterols (pharmacology)
• Lung Neoplasms (pathology)
• Membrane Potentials
• Microscopy, Fluorescence
• Mitochondria (drug effects, physiology)
• Reactive Oxygen Species