Abstract |
After exposure of type I Streptococcus pneumoniae to nitrosoguanidine, 13 temperature-sensitive (ts) mutants were selected that were restricted in capacity to form colonies on blood agar at 38 C. Whereas colony formation by the type I parent (ts+) was unaffected by a temperature of as high as 39 C, the ts mutants exhibited a spectrum of temperature sensitivity in which colony formation was inhibited significantly at 36 C, 37 C, 38 C, or 39 C. Growth of ts mutants at 38 C in broth was reduced or delayed relative to that of ts organisms under identical conditions. In general, there was a direct correlation between degree of temperature sensitivity and genetic stability. Mutants grown at a permissive temperature resembled the ts+ type I parent in colonial morphology and properties of alpha- hemolysis, bile solubility, optochin sensitivity, and antibiotic sensitivity. Moreover, in vitro studies indicated that the mutants retained capsules of immunochemically reactive type I capsular polysaccharide.
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Authors | C M Helms, M B Grizzard, B Prescott, L Senterfit, S Urmacher, G Schiffman, R M Chanock |
Journal | The Journal of infectious diseases
(J Infect Dis)
Vol. 135
Issue 4
Pg. 582-92
(Apr 1977)
ISSN: 0022-1899 [Print] United States |
PMID | 16064
(Publication Type: Journal Article)
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Chemical References |
- Culture Media
- Nitrosoguanidines
- Penicillins
- Erythromycin
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Topics |
- Bile
- Binding Sites, Antibody
- Culture Media
- Erythromycin
- Mutation
(drug effects)
- Nitrosoguanidines
(pharmacology)
- Penicillin Resistance
- Penicillins
- Solubility
- Streptococcus pneumoniae
(isolation & purification)
- Temperature
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