Differential chemical diagnosis of primary hyperoxaluria type II. Highly sensitive analysis of optical isomers of glyceric acid by GC/MS as diastereoisomeric derivatives.

Abstract

We established a separation method for the optical isomers of glyceric acid in urine by modifying the derivatization steps of the procedure used for the screening and diagnosis. The trimethylsilyl derivatization step in the mass screening procedure was replaced by O-acetyl-(+)-2-butylation, and the samples were analyzed under equivalent GC/MS conditions by capillary gas chromatography on a DB-5MS column. This method can be applied to cases that show a high urinary concentration of glyceric acid to obtain a differential diagnosis of primary hyperoxaluria type II and d-glyceric aciduria easily. l-Glyceric acid was also isolated from the urine of healthy controls as one of the main peaks.

• Authors: Yoshito Inoue, Toshihiro Shinka, Morimasa Ohse, Tomiko Kuhara
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 823 Issue 1 Pg. 2-6 (Aug 25 2005) ISSN: 1570-0232 [Print] Netherlands
• PMID: 16055048 (Publication Type: Journal Article)

Chemical References

• Glyceric Acids
• glyceric acid

Topics

• Diagnosis, Differential
• Gas Chromatography-Mass Spectrometry (methods)
• Glyceric Acids (analysis, chemistry)
• Humans
• Hyperoxaluria, Primary (diagnosis, urine)
• Male
• Reproducibility of Results
• Sensitivity and Specificity
• Stereoisomerism