HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Dynamic analysis of metabolic effects of chloroacetaldehyde and cytochalasin B on tumor cells using bioelectronic sensor chips.

AbstractPURPOSE:
To study the interplay of drugs and energy metabolism of tumor cells, metabolic changes induced by chloroacetaldehyde and cytochalasin B were analyzed in colon carcinoma cells LS174T.
METHODS:
O(2)-consumption and extracellular acidification were recorded using a bioelectronic sensor-chip system, which monitors these parameters in a culture continuously for at least 24 h. In parallel cultures cell number, cellular ATP-content, mitochondrial transmembrane potential, and the content of reactive oxygen species (ROS) were determined.
RESULTS:
When cell death was induced by chloroacetaldehyde (50 muM), the rate of acidification declined gradually for the next 15 h, while O(2)-consumption decreased rapidly within 30 min. This correlated with a loss in mitochondrial potential. However, cellular ATP-level showed a transient increase at 2 h; also ROS levels increased up to 6 h. In cells treated with cytochalasin B (2 muM), which inhibits glucose uptake, the rate of O(2)-consumption increased and the acidification activity dropped, even upon glutamine depletion. Mitochondrial membrane potential transiently increased after 1 h, while ATP-content decreased; there was no change in the level of ROS.
CONCLUSION:
The pattern of changes in basic energy metabolism differs with the type of cell death and growth inhibition involved in the cytotoxic action of two different drugs.
AuthorsE R Motrescu, A M Otto, M Brischwein, S Zahler, B Wolf
JournalJournal of cancer research and clinical oncology (J Cancer Res Clin Oncol) Vol. 131 Issue 10 Pg. 683-91 (Oct 2005) ISSN: 0171-5216 [Print] Germany
PMID16047190 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Reactive Oxygen Species
  • Cytochalasin B
  • chloroacetaldehyde
  • Acetaldehyde
Topics
  • Acetaldehyde (analogs & derivatives, pharmacology)
  • Adenocarcinoma (metabolism)
  • Biosensing Techniques (methods)
  • Cell Line, Tumor
  • Colorectal Neoplasms (metabolism)
  • Cytochalasin B (pharmacology)
  • Energy Metabolism (drug effects)
  • Humans
  • Membrane Potentials (drug effects)
  • Mitochondria (drug effects)
  • Oxygen Consumption (drug effects)
  • Reactive Oxygen Species (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: