Abstract | PURPOSE: METHODS: O(2)-consumption and extracellular acidification were recorded using a bioelectronic sensor-chip system, which monitors these parameters in a culture continuously for at least 24 h. In parallel cultures cell number, cellular ATP-content, mitochondrial transmembrane potential, and the content of reactive oxygen species (ROS) were determined. RESULTS: When cell death was induced by chloroacetaldehyde (50 muM), the rate of acidification declined gradually for the next 15 h, while O(2)-consumption decreased rapidly within 30 min. This correlated with a loss in mitochondrial potential. However, cellular ATP-level showed a transient increase at 2 h; also ROS levels increased up to 6 h. In cells treated with cytochalasin B (2 muM), which inhibits glucose uptake, the rate of O(2)-consumption increased and the acidification activity dropped, even upon glutamine depletion. Mitochondrial membrane potential transiently increased after 1 h, while ATP-content decreased; there was no change in the level of ROS. CONCLUSION: The pattern of changes in basic energy metabolism differs with the type of cell death and growth inhibition involved in the cytotoxic action of two different drugs.
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Authors | E R Motrescu, A M Otto, M Brischwein, S Zahler, B Wolf |
Journal | Journal of cancer research and clinical oncology
(J Cancer Res Clin Oncol)
Vol. 131
Issue 10
Pg. 683-91
(Oct 2005)
ISSN: 0171-5216 [Print] Germany |
PMID | 16047190
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Reactive Oxygen Species
- Cytochalasin B
- chloroacetaldehyde
- Acetaldehyde
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Topics |
- Acetaldehyde
(analogs & derivatives, pharmacology)
- Adenocarcinoma
(metabolism)
- Biosensing Techniques
(methods)
- Cell Line, Tumor
- Colorectal Neoplasms
(metabolism)
- Cytochalasin B
(pharmacology)
- Energy Metabolism
(drug effects)
- Humans
- Membrane Potentials
(drug effects)
- Mitochondria
(drug effects)
- Oxygen Consumption
(drug effects)
- Reactive Oxygen Species
(metabolism)
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