Acute
neuroprotective effects of
cinnamophilin (CINN; (8R, 8'S)-4, 4'-dihydroxy-3, 3'-dimethoxy-7-oxo-8, 8'-
neolignan), a novel
antioxidant and
free radical scavenger, were studied in a mouse model of transient middle cerebral artery (MCA) occlusion. CINN was administered intraperitoneally either 15 min before (pretreatment) or 2 h after the onset of MCA occlusion (postischemic treatment). Relative to vehicle-treated controls, animals pretreated with CINN, at 20-80 mg/kg, had significant reductions in
brain infarction by 33-46% and improvements in neurobehavioral outcome. Postischemic administration with CINN (80 mg/kg) also significantly reduced
brain infarction by 43% and ameliorated neurobehavioral deficits. Additionally, CINN administration significantly attenuated in situ accumulation of
superoxide anions (O2-) in the boundary zones of
infarct at 4 h after reperfusion. Consequently, CINN-treated animals exhibited significantly decreased levels of oxidative damage, as assessed by immunopositive reactions for
8-hydroxy-2'-deoxyguanosine (8-OHdG) and
4-hydroxynonenal (4-HNE), and the resultant inflammatory reactions at 24 h post-insult. It is concluded that CINN effectively reduced
brain infarction and improved neurobehavioral outcome following a short-term recovery period after severe transient focal
cerebral ischemia in mice. The finding of a decreased extent of
reactive oxygen species and oxidative damage observed with CINN treatment highlights that its
antioxidant and radical scavenging ability is contributory.