Abstract | BACKGROUND: Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent of adult T-cell leukemia (ATL), whereas HTLV type 2 (HTLV-2), is not associated with ATL or any other leukemia. HTLV-1 encodes the transforming gene tax1, whose expression in an interleukin (IL)-2-dependent T-cell line (CTLL-2) induces IL-2-independent growth. RESULTS: In this study, we demonstrated that IL-2-independent growth induction by Tax1 was abrogated by mutations of the PDZ domain-binding motif ( PBM) at the Tax1 C-terminus. HTLV-2 Tax2, which shares 75% amino acid identity with Tax1 but does not have a PBM, was not able to induce IL-2-independent growth of CTLL-2. CONCLUSION: Our results suggest that Tax1, through interaction with PDZ domain protein(s) induces IL-2-independent growth, which may be a factor in multi-step leukemogenesis caused by HTLV-1.
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Authors | Chikako Tsubata, Masaya Higuchi, Masahiko Takahashi, Masayasu Oie, Yuetsu Tanaka, Fumitake Gejyo, Masahiro Fujii |
Journal | Retrovirology
(Retrovirology)
Vol. 2
Pg. 46
(Jul 23 2005)
ISSN: 1742-4690 [Electronic] England |
PMID | 16042787
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Gene Products, tax
- Interleukin-2
- NF-kappa B
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Topics |
- Binding Sites
- Cell Line
- Cell Proliferation
- Gene Products, tax
(chemistry, physiology)
- Humans
- Interleukin-2
(physiology)
- Leukemia-Lymphoma, Adult T-Cell
(etiology)
- NF-kappa B
(physiology)
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