Abstract | OBJECTIVE: We sought to examine the expression and functional role of osteonectin in primary and metastatic pancreatic ductal adenocarcinoma (PDAC). BACKGROUND: METHODS: RESULTS: There was a 31-fold increase in osteonectin mRNA levels in PDAC and a 16-fold increase in chronic pancreatitis as compared with the normal pancreas (P < 0.01). By immunohistochemistry, faint immunoreactivity was detected in the normal pancreas. In contrast, strong staining of the cancer cells was observed in addition to extensive osteonectin immunoreactivity in surrounding fibroblasts and in the extracellular matrix. In metastatic tissues, strong immunoreactivity was observed in fibroblasts and in extracellular matrix surrounding metastatic cancer cells, whereas the signal was absent in most tumor cells. In vitro studies showed that osteonectin was able to inhibit cancer cell growth while promoting invasiveness of pancreatic tumor cells. CONCLUSION:
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Authors | Ahmed Guweidhi, Jörg Kleeff, Hassan Adwan, Nathalia A Giese, Moritz N Wente, Thomas Giese, Markus W Büchler, Martin R Berger, Helmut Friess |
Journal | Annals of surgery
(Ann Surg)
Vol. 242
Issue 2
Pg. 224-34
(Aug 2005)
ISSN: 0003-4932 [Print] United States |
PMID | 16041213
(Publication Type: Journal Article)
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Chemical References |
- Oligonucleotides, Antisense
- Osteonectin
- RNA, Messenger
- Recombinant Proteins
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Topics |
- Adenocarcinoma
(genetics, mortality, pathology)
- Adult
- Aged
- Disease Progression
- Enzyme-Linked Immunosorbent Assay
- Extracellular Matrix
(chemistry)
- Female
- Fibroblasts
(chemistry)
- Humans
- Immunohistochemistry
- Male
- Middle Aged
- Neoplasm Invasiveness
(physiopathology)
- Oligonucleotides, Antisense
(pharmacology)
- Osteonectin
(analysis, genetics, physiology)
- Pancreatic Neoplasms
(genetics, mortality, pathology)
- Pancreatitis
(metabolism)
- Polymerase Chain Reaction
- Prognosis
- RNA, Messenger
(analysis)
- Recombinant Proteins
(pharmacology)
- Tumor Cells, Cultured
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