Abstract | RATIONALE: Clearance of apoptotic cells is crucial to the resolution of inflammation and development of fibrosis, but the process is not well understood in normal or diseased human lungs. OBJECTIVES: To determine phagocytosis of apoptotic cells by primary human alveolar macrophages and whether defects in uptake of apoptotic cells are associated with decreases in antiinflammatory/antifibrotic mediators. METHODS: Human bronchoalveolar lavage macrophages ( AMphis) from normal control subjects and subjects with mild-moderate or severe asthma were examined in vitro for phagocytosis of apoptotic human T-cell line Jurkats and secretion of inflammatory mediators. MEASUREMENTS AND MAIN RESULTS: CONCLUSIONS: A decrease in AMphis LPS-responsiveness in severe asthma is manifested by defective apoptotic cell uptake and reduces secretion of inflammatory mediators. This may contribute to the chronicity of inflammation and remodeling in lungs of patients with asthma.
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Authors | Mai-Lan N Huynh, Kenneth C Malcolm, Chakradhar Kotaru, John A Tilstra, Jay Y Westcott, Valerie A Fadok, Sally E Wenzel |
Journal | American journal of respiratory and critical care medicine
(Am J Respir Crit Care Med)
Vol. 172
Issue 8
Pg. 972-9
(Oct 15 2005)
ISSN: 1073-449X [Print] United States |
PMID | 16040786
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents
- Hydroxyeicosatetraenoic Acids
- Tumor Necrosis Factor-alpha
- Dexamethasone
- 15-hydroxyeicosatrienoic acid
- Granulocyte-Macrophage Colony-Stimulating Factor
- Dinoprostone
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Topics |
- Adult
- Analysis of Variance
- Anti-Inflammatory Agents
(immunology, therapeutic use)
- Apoptosis
(immunology)
- Asthma
(classification, diagnosis, immunology)
- Bronchoalveolar Lavage Fluid
- Case-Control Studies
- Cell Survival
- Chronic Disease
- Dexamethasone
(immunology, therapeutic use)
- Dinoprostone
(analysis, immunology)
- Female
- Forced Expiratory Volume
- Granulocyte-Macrophage Colony-Stimulating Factor
(immunology)
- Humans
- Hydroxyeicosatetraenoic Acids
(analysis, immunology)
- Inflammation
- Jurkat Cells
- Macrophages, Alveolar
(immunology)
- Male
- Phagocytosis
(immunology)
- Severity of Illness Index
- Tumor Necrosis Factor-alpha
(immunology)
- Vital Capacity
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