Abstract | BACKGROUND: MATERIALS AND METHODS: Genomic DNA was isolated from two cell lines from metastatic prostate adenocarcinoma in heterotransplanted male athymic nude (nu/nu) Balb/c mice. Mutation screening was performed by sequencing of exons 1-8 of the human androgen receptor gene. RESULTS: Despite two polymorphisms found in the transactivation domain of hAR exon 1, no point mutations were detected in the hAR gene of both cell lines. CONCLUSION: Point mutations of hAR are not necessary for metastatic prostate cancer, while alterations in the solyglutamine and polyglycine repeat region in exon 1 of the MR gene are more often found. These repeats are two of many genetic influences that contribute to the overall risk of developing prostate cancer.
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Authors | Dirk G Engehausen, F Steffen Krause, Johann Fleischmann, Ziya Akcetin, Karl M Schrott, Sabine Endele |
Journal | Anticancer research
(Anticancer Res)
2005 May-Jun
Vol. 25
Issue 3A
Pg. 1611-4
ISSN: 0250-7005 [Print] Greece |
PMID | 16033069
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA Primers
- Receptors, Androgen
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Topics |
- Animals
- Base Sequence
- Cell Line, Tumor
- DNA Primers
- Humans
- Male
- Mice
- Mice, Nude
- Neoplasm Transplantation
- Point Mutation
- Polymorphism, Genetic
- Prostatic Neoplasms
(genetics, pathology)
- Receptors, Androgen
(genetics)
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