Skin carcinomas and micronucleus induction in epidermal keratinocytes following 12-O-tetradecanoylphorbol-13-acetate and mezerein treatment.

Abstract

Phorbol esters are chemical compounds which have the ability to promote carcinogenesis in the skin of mice. In the HRA/Skh mouse strain, an initiation-promotion assay substantiated evidence that mezerein acts as a complete promoter. Complete promotion with 9.0 nmol mezerein induced a total of 53 papillomas in 28 mice within 40 weeks, while 1.5 nmol 12-O-tetradecanoylphorbol-13-acetate (TPA), using the same application regime, resulted in a total of 298 papillomas in 58 mice. However, 7.6% of the papillomas in the mezerein group progressed to carcinomas, compared with 0.7% in the TPA group. Approximately the same molar concentrations of TPA and mezerein induced a dose-dependent and reproducible increase in micronuclei in supra-basal cells derived from the epidermis of treated mice. In contrast, the latter compound was less toxic to these cells and induced higher numbers of micronuclei at the higher concentrations.

• Authors: H H Steinel, A M Bonin, K Meher-Homji, R S Baker
• Journal: Mutagenesis (Mutagenesis) Vol. 7 Issue 3 Pg. 199-203 (May 1992) ISSN: 0267-8357 [Print] England
• PMID: 1602975 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carcinogens
• Diterpenes
• Terpenes
• mezerein
• Tetradecanoylphorbol Acetate

Topics

• Animals
• Carcinogens (toxicity)
• Diterpenes
• Female
• Keratinocytes (cytology, drug effects)
• Kinetics
• Mice
• Micronucleus Tests
• Skin Neoplasms (chemically induced)
• Terpenes (toxicity)
• Tetradecanoylphorbol Acetate (pharmacology)